条斑紫菜叶状体DNA提取方法的研究  

Study on Methods of DNA Extraction from Thallus of Porphyra yezoensis

作　　者：王萍[1] 倪伟镯 孟学平[1] 

机构地区：[1]淮海工学院海洋学院江苏省海洋生物技术重点建设实验室,连云港222005

出　　处：《吉林农业大学学报》2007年第6期640-642,646,共4页Journal of Jilin Agricultural University

基　　金：江苏省海洋生物技术重点建设实验室开放课题资助项目(2005HS011);淮海工学院引进人才科研启动基金资助项目(KK01073)

摘　　要：以常用的植物基因组DNA提取方法—LiCl法、SDS法、CTAB法、高盐脲法和试剂盒法提取条斑紫菜叶状体DNA,对不同提取方法的效果进行了比较分析,同时探讨了SDS法的细胞破碎方法和不同样本量提取条斑紫菜DNA的效果。对DNA纯度、DNA浓度、DNA得率等分析表明,SDS法效果较好,其次是LiCl法;在使用SDS法时,液氮研磨破碎细胞效果好于其他方法;在小样本量提取DNA时,SDS法比LiCl法效果好。DNA from thallus of Porphyra yezoensis was extracted by method of LiCI, SDS, CTAB, Highsalt Urea and Plant Genomic DNA Isolation Kit, and the effects of different extraction methods were compared and analyzed. At the same time, the effects of smudge cell methods and differrent sample sizes to extract the genomic DNA of Porphyra yezoensis were discussed. By evaluating purification of DNA, concentration of DNA, and DNA yield, the results indicated that the effect of SDS was better than other methods, and then was LiCl. When SDS was used, the smudge cell by skive with liquid nitrogen had the best effect among these methods. SDS to extract DNA was better than LiCl in small sample.

关 键 词：条斑紫菜 叶状体 DNA提取 

分 类 号：Q78[生物学—分子生物学] Q949.321