漆酶固定条件的实验研究  

Experimental study of immobilized laccase

作　　者：周菊英[1] 徐柳苏[1] 古家虹[1] 盘荣俊[1] 

机构地区：[1]广西工学院信息与计算科学系,广西柳州545006

出　　处：《广西工学院学报》2007年第3期1-3,7,共4页Journal of Guangxi University of Technology

基　　金：国家自然科学基金(No.60377032)项目资助

摘　　要：制备了四氨基酞菁铜(CuTAPc)-Fe3O4纳米复合粒子,以戊二醛作为交联剂,成功地将漆酶固定在CuTAPc-Fe3O4纳米复合粒子上。探讨了各种因素对固定化漆酶活力的影响。结果表明:当以10%的戊二醛与载体交联8h后,再以2 mg/mL的漆酶溶液(pH=7)于4℃下固定所得固定化漆酶活力最高。CuTAPc-Fe3O4 nanoparticles composites were prepared, using glutaradehyde as cross-linking , laccase has been succesfully immobilized on copper tetraaminophthalocyanine-ferrorerric oxide （CuTAPc-Fe3O4）. The immobilization conditions were investigated. The results showed the optimal conditions were： 15mg nanoparti- cles reacted with 10% glutaradehyde for 8 hours and subsequently reacted with 2mg/mL laccase in pH 7.0 phos- phate buffer at 412 for 24h. Under these conditions, the immobilized laccse has the best activity.

关 键 词：CuTAPc-Fe3O4 固定 固定化漆酶 活性 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]