Nd^(+3): YAG激光对黑曲霉的诱变效应  

Mutagenic Effects of Nd +3 :YAG Laser on Aspergillus Niger

作　　者：杨胜利[1] 赖素聪[1] 杨楝 赖葆青 朱建忠 李如鹤 

机构地区：[1]厦门大学

出　　处：《激光生物学报》1997年第2期1071-1073,共3页Acta Laser Biology Sinica

摘　　要：本文采用Ｎｄ＋３：ＹＡＧ激光辐照柠檬酸生产菌黑曲霉孢子，辐照后进行培养和发酵试验、分析测定不同辐照时间黑曲霉孢子的存活率、黑曲霉菌丝体生长繁殖及代谢产酸的速度、主要代谢产物柠檬酸的产量及淀粉糖化酶活力等的变化。实验结果表明，Ｎｄ＋３：ＹＡＧ激光辐照后各实验组发生了不同程度的变化，说明该激光能诱发黑曲霉细胞的遗传变异。In this paper, a Nd +3 :YAG laser was used to irradiate spores of the Aspergillus niger, a citric acid-producing strain. The spores irradiated were then cultured and fermentated. Variations in a survival rates of the spores, rates of growth, propagation, and the metabolic production acid of the hyphae, yield of the main metabolite citric acid and vitality of the saccharidase, etc . were determined and analysed. The experimental results show that the various experimental groups, irradiated by the Nd:YAG laser, had changes in different degrees. This indicates that the laser can induce Aspergillus niger cells to mutate and may possibly be applied to selectively breed new good quality strains with high yield for production of the citric acid.

关 键 词：黑曲霉 激光辐照 诱变效应 Ad^+3:YAG激光 发酵 

分 类 号：Q949.327.1[生物学—植物学] Q631