影响农杆菌转化效率的植物因子H2A1基因的克隆、序列分析及其植物表达载体的构建  

Cloning of H2A1,a Plant Factor Influencing Agrobacterium-mediated Transformation and Constructing of a Plant Expression H2A1 Vector

作　　者：邹智[1] 吴刚[1] 卢长明[1] 

机构地区：[1]中国农业科学院油料作物研究所农业部油料作物遗传改良重点开放实验室,武汉430062

出　　处：《生物技术通报》2007年第6期83-87,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30471009)

摘　　要：拟南芥组蛋白H2A1是影响农杆菌T-DNA整合到宿主基因组的关键因素之一。与其它H2A组蛋白一样,它含有1个保守的H2A结构域。本研究利用PCP技术从拟南芥中成功扩增到H2A1基因组序列的全长。产物纯化后克隆到pBI121(Kmr),从而构建了H2A1的植物表达载体pBI121-H2A1。PCR和DNA测序证实载体构建成功。最后用电击法将该重组质粒导入根癌农杆菌LBA4404菌株中形成工程菌。此表达载体的构建为进一步研究H2A1的功能和提高外源基因在植物中稳定表达水平奠定了基础。H2A1, a protein of Arabidopsis thaliana,is a key factor influencing T-DNA intergration of Agrobacterium. Like other Histones,it has a conserved H2A motif. The genomic DNA sequence of H2A1 was amplified by PCR（polymerase chain reaction）and subcloned into pBI121 to form a plant expression vector called pBI121-H2A1,as proved by PCR analysis and sequencing. Then the recombination plasmid was electroporated into Agrobacterium tumefaciens strain LBA4404 to form an engineering strain. This work lays a basis for clarifying the function of H2A1 gene and increasing the stable expression level of foreign DNAs.

关 键 词：H2A1植物因子 根癌农杆菌 遗传转化 植物表达载体 

分 类 号：Q943.2[生物学—植物学]