两种滨藜甜菜碱醛脱氢酶基因的克隆及序列分析  

Cloning and Sepuence Analysis of Betaine Aldehyde Dehydrogenase in Atriple

作　　者：朱先灿[1] 胡鸢雷[1] 王晓丽[1] 祝建波[2] 林忠平[1] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]新疆石河子大学绿洲生态农业重点实验室,石河子832003

出　　处：《生物技术通报》2007年第6期88-91,共4页Biotechnology Bulletin

摘　　要：甜菜碱醛脱氢酶(Betaine aldehyde dehydrogenase,BADH)对非生物胁迫下植物渗透调节物质的合成和积累具有重要作用。分别从异苞滨藜和鞑靼滨藜两种盐生植物中分离到了BADH基因。序列分析表明,BADH全长均为1 507bp,编码501个氨基酸,两种BADH序列具有较高的相似性。甜菜碱醛脱氢酶的克隆为植物的基因转化及其功能分析奠定了基础。Betaine aldehyde dehydrogenase （BADH）plays a vital role of osmotic adjustment substances synthesize and accumulate by abiotic stresses.The BADH genes were amplified from Atriplex micrantha C. A. Mey and Atriplex tatarica L through PT-PCR technique.sequence analysis shows BADHs both were 1 507bp in full length,and encoding 501 amino acid.The two BADH have high comparability.Cloning of BADH may set up a solid foundation for giving further insight on plant transgenetic and function analysis.

关 键 词：异苞滨藜 鞑靼滨藜 BADH 基因克隆 序列分析 

分 类 号：Q943.2[生物学—植物学]