萝卜花药培养再生植株染色体倍数FCM分析及田间观察  

Flow Cytometric Analysis of Ploidy Level of Regenerated Plants Obtained by Anther Culture in Radish(Raphanus sativus L.)and Field Observation

作　　者：熊秋芳[1] 张雪清[1] 荣凯峰[2] 

机构地区：[1]武汉市蔬菜科学研究所 [2]武汉工程大学生物技术学院

出　　处：《中国蔬菜》2007年第12期19-21,共3页China Vegetables

基　　金：武汉市科技局晨光计划(20065004116-45)

摘　　要：采用流式细胞仪(FCM)对萝卜的20株花药培养再生植株进行倍性鉴定。结果显示:流式细胞仪清楚地显示了每份材料的DNA含量,其中单倍体、二倍体、四倍体、嵌合体的比例分别为20%、30%、25%、25%,并对照再生植株田间生长情况,从形态学角度验证了FCM分析的准确性。Flow cytometic（FCM） analysis of ploidy level was made with twenty radish regenerated plants obtained by anther culture. Numbers of haploid, diploid, tetra-ploid and chimera were learned from the DNA content of the regenerated plants tested. In the analysis, haploids, diploids, tetra-ploids and chimeras covered 20 % ,30 % ,25 % and 25 % respectively. The ploidy levels of regenerated plants were identical to that of the plants grown in the field.

关 键 词：萝卜 花药培养 FCM分析 田间观察 

分 类 号：S631.1[农业科学—蔬菜学]