泻火补肾汤对脑出血神经干细胞移植后大鼠脑内VCAM-1、TGF-β_1基因表达的影响  

Effect of Xiehuobushen Decoction on the Gene Expressions of TGF-β_1 and VCAM-1 in Rats Brain of Neural Stem Cells-transplanted Intracerebral Hemorrhage

作　　者：刘清娥[1] 邢之华[1] 黎杏群[1] 罗杰坤[1] 钟鹏程[1] 罗文芳[1] 刘笑迎[2] 

机构地区：[1]中南大学湘雅医院,长沙410008 [2]上海中医药大学附属岳阳医院,上海200437

出　　处：《中国中医急症》2007年第12期1501-1503,1521,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No30472264)

摘　　要：目的研究泻火补肾汤(XHBSD)对神经干细胞(NSCs)移植入脑出血大鼠脑室下区后血管细胞间黏附分子(VCAM-1)和转化生长因子(TGF-β1)基因表达的影响,探讨泻火补肾汤能否诱导免疫耐受。方法SD大鼠随机分为六A(正常组)5只,B(假手术组)、C(模型组)、D(模型治疗组)、E(移植组)、F(移植治疗组)各25只。用RT-PCR方法观察各组术后第2、4、7、14、28日TGF-β1 mRNA及VCAM-1 mRNA的表达。结果B、C、D、E、F五组VCAM-1 mRNA和TGF-βl mRNA分别在第2、4日表达达高峰(P<0.05)。VCAM-1 mRNA在各时间点E组表达最强(P<0.05),而TGF-1 mRNAF组表达最强(P<0.01)。同时间点除第28日外,F组比E组、D组比C组TGF-β1 mRNA表达增强(P<0.05),而VCAM-1 mRNA表达减弱(P<0.05)。结论泻火补肾汤可通过促进TGF-β1 mRNA的表达,降低VCAM-1 mRNA的表达,有助于诱导免疫耐受。Objective： To observe the effect fo Xiehuobushen Decoction（XHBSD） on the gene expressions of transforming growth factor -betal（TGF - β1 ）and vascular cell adhesion molecular - 1 （VCAM - 1 ）in the rats brain of NSCs - transplanted intracerebral hemorrhage, in order to investigate whether the XHBSD can induce immunotolerance or not. Methods： One hundred and thirty Sprague - Dawley Rats were randomly divided into six groups： A group （normal group）（ n =5）, B group （sham operation group）（ n =25）, C group （model group）（ n =25）, D group（treated model group） （ n = 25）, E group（transplanted group） （ n = 25）, F group（treated transplanted - group） （ n = 25）. The expressions ofTGF- β1 mRNA and VCAM - lmRNA were assayed by RT -PCR at 2d, 4d, 7d, 14d, 28d after the operation. Results： The expressions of VCAM -1mRNA and TGF - β1 mRNA in B, C, D, E, F groups arrived the peak at 2nd day and 4d respectively, The expression of VCAM - 1mRNA in E group was the highest among all the groups, while the expression of TGF - β1 mRNA in F group was the highest . At the same time except 28th day, the expression of TGF - β1 mRNA in F and D groups were significantly higher than E and C groups respectively, and the expression of VCAM - 1mRNA in F and D groups were lower than E and C groups respectively. Conclusion： XHBSD can promote the expression of TGF - β1 mRNA and inhibit the expression of VCAM - 1mRNA in the rats brain of NSCs - transplanted intracerebral hemorrhage, and may induce the immunotolerance of the receptor.

关 键 词：神经干细胞 移植 免疫耐受 血管细胞间黏附分子 转化生长因子 脑出血 

分 类 号：R285.5[医药卫生—中药学]