神经生长因子对穹窿海马伞切割后海马自体神经干细胞增殖和向神经元分化的影响  

THE EFFECTS OF NGF ON THE PROLIFERATION AND DIFFERENTI-ATION OF ENDOGENOUS NSCs IN THE HIPPOCAMPUS INTO NEURONS AFTER FIMBRIA FORNIX TRANSECTION

作　　者：董传明[1] 金国华[1] 秦建兵[1] 田美玲[1] 邹琳清[1] 谭雪锋[1] 朱蕙霞[2] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所江苏省神经再生重点实验室,南通226001 [2]南通大学医学院人体解剖学教研室神经生物学研究所生物化学教研室,南通226001

出　　处：《解剖学报》2007年第6期642-646,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30670648);江苏省自然科学基金资助项目(BK2006057)

摘　　要：目的探讨切割穹窿海马伞及脑室给予神经生长因子(NGF)后,对大鼠海马自体神经干细胞增殖和分化为神经元的影响。方法12只SD大鼠,随机分成给药组和对照组,每组6只,切割右侧穹窿海马伞,两组切割后即时及第2d、4d向侧脑室分别注射NGF和人工脑脊液,并在术后第3～7d每日腹腔注射2次BrdU。于术后28d灌注取脑、冷冻切片,行BrdU/NF-200免疫荧光双标检测。结果海马齿状回内BrdU阳性细胞数,给药组和对照组切割侧明显多于正常侧,给药组切割侧和正常侧分别多于对照组切割侧和正常侧;海马齿状回内的BrdU/NF-200双标神经元数,给药组切割侧最多,给药组正常侧和对照组切割侧较少,而对照组正常侧则无。结论切割穹窿海马伞后,可致大鼠海马齿状回自体神经干细胞增殖加快并向神经元分化,脑室施加NGF可促进其增殖和分化。Objective To study the effects of fimbria fornix transection and intraventricular injection of nerve growth factor（NGF） on the proliferation and differentiation of endogenous neural stem cells （NSCs）in the hippocampus into neurons. Methods Twelve SD rats were divided into the treatment group and control group randomly, six rats in each group. The right fimbria fornix of rats were transected. NGF and artificial cerebrospinal fluid was injected into the lateral ventricle of rats in the treatment group and control group respectively immediately and on the 2 nd and 4 th day after transection. Bromodeoxyuridin （BrdU） was injected intraperitoneally twice per day from 3 to 7 days after operation. On the 28 th day after operation, the brains were perfused for fixation and frozen section. BrdU/NF-200 double-label immunofluorescence was used to detect the proliferation and differentiation of NSCs into neurons. Results In dentate gyms, the number of BrdU positive cells in the transection side was much more than those of normal side both in the treatment group and the control group, and the BrdU positive cells in treatment group were more than those of control group both in the transection side and normal side; The number of BrdU/NF-200 double labeled neurons was the most in the transection side of the treatment group, less in normal side Gf treatment group and transection side of the control group, but absent in the normal side of the control group. Conclusion Fimbria fornix transection could cause endogenous neural stem cells in the hippocampus proliferate and differentiate into neurons, and intraventricular injection of NGF could facilitate its proliferation and differentiation.

关 键 词：神经生长因子 穹窿海马伞切割 海马 神经干细胞 增殖 分化 免疫荧光 大鼠 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]