人肝干细胞移植增加四氯化碳诱导性肝硬化大鼠白蛋白表达  

TRANSPLANTATION OF HUMAN HEPATIC STEM CELLS INCREASED THE EXPRESSION OF ALBUMIN IN HEPATOCIRRHOSIS RATS INDUCED BY CARBON TETRACHLORIDE

作　　者：王晓红[1] 张立英[1] 张艳[1] 黄慧雅[1] 孔令英[1] 张书永[1] 沈丽[2] 张卫光[1] 

机构地区：[1]北京大学医学部解剖学与组织学胚胎学系,北京100083 [2]北京大学干细胞研究中心,北京100083

出　　处：《解剖学报》2007年第6期671-674,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30170244;30471638);北京市自然科学基金资助项目(7062041)

摘　　要：目的旨在研究人胚胎肝干细胞移植入四氯化碳(CCl4)诱导性肝硬化大鼠的肝组织后,大鼠血清及肝组织血清白蛋白(ALB)表达的变化情况。方法将健康雄性Wistar大鼠随机分为对照组和肝硬化组。对照组背部皮下注射橄榄油,CCl4组背部皮下注射CCl4橄榄油。连续注射6周后,肝硬化组再随机分为肝干细胞假移植组和肝干细胞移植组。对照组和假移植组在大鼠肝中叶注射细胞培养液,而移植组则在相应部位注射人胚胎肝干细胞2×106,移植4周后处死所有大鼠。右心室取血离心观察ALB的含量;肝中叶取材,免疫组织化学染色观察人表皮生长因子受体(EGFR)的表达,免疫荧光染色和免疫印迹法(Western blotting)法观察和测定肝组织ALB的表达变化。结果对照组和肝干细胞假移植组未见人EGFR阳性表达的细胞,肝干细胞移植组则可见大量排列成环状的EGFR阳性表达的细胞。在肝干细胞移植组的血清ALB含量、免疫荧光染色及Western blotting法检测肝组织ALB表达,均明显高于假移植组,而与对照组无明显著异。结论将人胚胎肝干细胞移植入肝硬化大鼠肝内可以长期存活,并可改善大鼠ALB的表达和分泌。Objective To study the change of the contents of albumin （ALB） in blood serum and liver tissue after human embryo-hepatic stem cells transplanted into hepatocirrhosis rats induced by CCl4. Methods Healthy mature male Wistar rats was randomly divided into control group and hepatocirrhosis group. The control group was injected oily solution with 0. 12ml/100g rat weight, the hepatocirrhosis group was subcutaneously injected 60% carbon tetrachloride oily solution with 0. 3ml/100g rat weight. Six weeks after injection, hepatocirrhosis group was subdivided into hepatic stem cells sham group and hepatic stem cells transplanted group. The control group and hepatic stem cells sham group was injected culture media, and hepatic stem cells transplanted group was injected human embryo-hepatic stem cells 2 × 10^6 for each rat. On the first day of 11 th weeks, all rats were killed. Blood samples were collected from right ventricles and the contents of ALB in serum of each sample was measured. Observed the expression of human EGFR by immunohistochemistry stain. The expressions of ALB in liver tissue was detected by immunofluorescence and Western-blotting. Results In the control and hepatic stem cells sham group, there was no expression of human epidermal growth factor receptor（EGFR）, but in hepatic stem cells transplanted group, there were lots of human EGFR positive cells. In hepatic stem cells transplanted group, but in liver tissue and blood serum the contents of ALB were more than that of hepatic stem cells sham group, but similar to the control group. Conclusion In carbon tetrachloride induced hepatocirrhosis rats, transplanted hepatic stem cells could survive long, and could improve the expression and secretion of ALB in rat liver tissue.

关 键 词：肝硬化 人胚胎肝干细胞 四氯化碳 白蛋白 免疫印迹法 大鼠 

分 类 号：R332[医药卫生—人体生理学]