低剂量棉酚甲基睾丸酮和炔雌醇联合用药作为男性避孕药的安全性检测  

SAFETY TEST OF GOSSYPOL PLUS METHYLTESTOSTERONE AND ETHINYLESTRADIOL AS A MALE CONTRACEPTIVE

作　　者：崔光辉[1] 钱晓菁[1] 许增禄[1] 仇文颖[1] 徐园园[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院人体解剖学与组织学胚胎学系,北京100005

出　　处：《解剖学报》2007年第6期713-717,共5页Acta Anatomica Sinica

摘　　要：目的探讨低剂量棉酚12mg/(kg·d)、甲基睾丸酮20mg/(kg·d)和炔雌醇100μg/(kg·d)联合用药24周,能否影响大鼠生精干细胞的功能。方法采用生精细胞原位凋亡、生精干细胞移植和生殖能力自主恢复实验。结果联合用药24周后,在大鼠睾丸组织切片中发现了凋亡信号阳性细胞,凋亡信号阳性细胞主要是精母细胞和精子细胞,没有发现精原细胞出现凋亡阳性信号。当把用药组大鼠的生精干细胞移植进入受体裸鼠的曲细精管内2～3个月后,在受体裸鼠的曲细精管中观察到了来自供体大鼠的长型精子细胞。停止用药6周后,用药组大鼠的生殖能力与对照组相比完全恢复。由恢复生殖能力的用药组大鼠产生的F1代仔鼠在经过一系列的检测后被证明为身体机能和智力水平正常。结论此联合用药在24周内没有影响到用药大鼠生精干细胞的生存和分化功能。Objective To investigate whether administration of gossypol of 12mg/kg combined with methyhestosterone 20mg/kg and ethinylestradiol 100μg/kg daffy for a long term of 24 weeks could affect rat spermatogonial stem cell function. Methods TdT-mediated dUTP nick end-labeling （TUNEL） detection, spermatogonial stem cell transplantation and fertility recovery evaluation were carried out. Results The administration of this regimen could induce germ cell going apoptosis; the apoptotic signal positive cells were spermatocyte and spermatid, no spermatogonium was found to be signal positive. Two to three months after transplantation of medicine treated spermatogonial stem cells, elongated rat spermatids were identified in the seminiferous tubules of recipient nude mice. Six weeks after withdrawal of the administration, fertility of the medicine treated rats was recovered completely compared with that of the control group. After a series of tests, litters of the first generation showed to be normal. Conclusion These results indicated that the administration of this regimen would not affect the function of rat spermatogonial stem cells.

关 键 词：棉酚 甾体激素 生精干细胞 移植 生殖能力 恢复 大鼠 

分 类 号：R979.21[医药卫生—药品]