水通道蛋白1在生后小鼠精囊腺与前列腺发育过程中的表达与分布  

EXPRESSION AND LOCALIZATION OF AQUAPORIN 1 IN MOUSE SEMINAL VESICLE AND PROSTATE GLAND DURING POSTNATAL DEVELOPMENT

作　　者：吕丹瑜[1] 李英[2] 毕振伍[2] 于和鸣 李学军[4] 

机构地区：[1]中国科学院研究生院,北京100049 [2]北京大学基础医学院组织学与胚胎学教研室,北京100083 [3]国家人口计划生育委员会科学技术研究所,北京100081 [4]北京大学基础医学院药理学系,北京100083

出　　处：《解剖学报》2007年第6期722-726,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金(39770286;30171090;30572202)资助;国家973项目基金(2004CB518902)资助;科研院所社会公益研究专项基金(2004DIB1J041)资助

摘　　要：目的探讨水通道蛋白1(AQP1)在雄性小鼠出生后不同发育阶段精囊腺和前列腺的表达与分布。方法应用RT-PCR、免疫印迹和免疫组织化学染色方法,检测出生后15d、35d、70d小鼠精囊腺、前列腺AQP1 mRNA、蛋白表达水平及细胞定位。结果RT-PCR与免疫印迹结果均显示,出生后70d小鼠精囊腺、前列腺AQP1 mRNA及蛋白表达量较出生后15d显著增强(P<0.05);免疫组织化学染色显示,AQP1蛋白仅表达于出生后15d、35d小鼠精囊腺平滑肌细胞、前列腺腺泡上皮细胞基膜及间质细胞,而此时期精囊腺上皮细胞AQP1蛋白表达呈阴性;出生后70d,AQP1蛋白强烈表达于精囊腺和前列腺上皮细胞。结论AQP1 mRNA及蛋白在雄性小鼠精囊腺、前列腺的表达与分布随年龄增长而改变,此变化可能与雄性附属性腺发育相关。Objective To examine the expression and distribution of Aquaporin 1 （AQP1） in the seminal vesicle and prostate gland of male mice during postnatal development. Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out. Results The results of RT-PCR and Western blotting showed that the level of AQP1 mRNA and protein were significantly increased in the seminal vesicle and prostate gland on postnatal day 70 （P 〈 0.05）. The positive signals of AQP1 protein were localized immunohistochemically in the smooth muscle cells of seminal vesicle and the basement membrane of glandular epithelium and the mesenchyme cells of prostate gland on postnatal day 15 and day 35, but it was hardly detected in the epithelium of seminal vesicle. Thereafter, AQP1 protein was intensively expressed in the epithelium of seminal vesicle and prostate gland on postnatal day 70. Conclusion The different expressions and distributions of AQP1 mRNA and protein in the seminal vesicle and prostate gland of mice in different ages may be related to in the development of male accessory glands.

关 键 词：水通道蛋白1 精囊腺 前列腺 生后发育 逆转录聚合酶链式反应 免疫印迹 免疫组织化学 小鼠 

分 类 号：Q492.4[生物学—生理学]