抗人精子甘露糖受体抗血清对精子穿透实验的影响  

EFFECTS ON SPERM OOCYTE PENETRATING BY ANTI SERUM TO HUMAN SPERM MANNOSE RECEPTOR

作　　者：江澍[1] 林典梁[1] 江一平[1] 

机构地区：[1]福建医科大学细胞与发育工程研究中心

出　　处：《解剖学报》2007年第6期727-730,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金(30271371);福建省自然科学基金(Z0516042);福建省教育厅科技项目(JA03124)资助

摘　　要：目的研究人精子甘露糖受体(MR)在精卵融合中的作用。方法用亲合层析法分离纯化的人精子甘露糖受体来免疫Balb/c小鼠得到抗MR抗血清;用抗血清处理顶体反应后的人精子,并进行去透明带金黄地鼠卵的精子穿透实验(SPA),观察其体外受精情况。结果抗MR抗血清可抑制人精子与去透明带金黄地鼠卵的结合与穿透,使卵子的受精率下降,具剂量依赖性。结论人精子MR与精卵膜的识别与融合有关。Objective To study the effect of mannose receptor（MR） from human spermatozoa on sperm-egg fusion. Methods The Balb/c mouse was immunized with the purified mannose receptor（pMR） which was isolated from motile human sperm by mannose-agarose gel affinity chromatography and the anti-MR serum was harvested. Human sperm after induction of acrosome reaction was pretreated with anti-MR serum and subsequently processed in sperm penetration assay （SPA）. Results The anti-MR serum can inhibit human spermatozoa from binding and penetrating zona-free golden hamster eggs with a dosedependent reduction of Fertilization rate （FR） and Penetration index （ PI）. Conclusion The results indicate that MR of human sperm plays an important role in sperm-oocyte fusion.

关 键 词：甘露糖受体 人精子 精卵融合 受精 金黄地鼠卵 

分 类 号：R321.2[医药卫生—人体解剖和组织胚胎学]