围着床期小鼠胚卵L-选择素的表达变化及作用  

EXPRESSION AND EFFECT OF L-SELECTIN IN MICE OVIGERMS DURING THE PERI-IMP LANTATION PERIOD

作　　者：王颢[1] 魏莎莉[1] 杨戎[1] 杨洁玉[1] 李南[1] 刘孝云[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生理学教研室,重庆400016

出　　处：《解剖学报》2007年第6期736-740,共5页Acta Anatomica Sinica

基　　金：重庆市教委课题资助(KJ050306)

摘　　要：目的探讨围着床期小鼠胚卵L-选择素的表达规律及其对胚胎着床的影响。方法1.分别采用实时荧光定量PCR(FQ-PCR)和免疫荧光组织化学方法检测围着床期小鼠胚卵L-选择素mRNA和蛋白的表达,并用免疫荧光组织化学方法检测L-选择素在细胞的定位;2.用子宫角内抗体干预的方法观察并统计小鼠着床胚胎数。结果1.随着胚卵分化成熟,L-选择素mRNA和蛋白表达均呈逐渐增加趋势,胚泡期表达最强(P<0.05)。L-选择素在单细胞期和4细胞期有少量表达,桑椹胚周边区L-选择素表达显著增强,胚泡滋养层L-选择素表达最强。2.小鼠子宫角实验侧(经抗L-选择素单克隆抗体干预)胚胎着床数较对照侧(经等体积生理盐水干预)明显减少(P<0.05)。结论胚卵可能通过L-选择素信号转导途径及其与子宫内膜的糖蛋白受体结合参与胚胎着床。Objective To investigate the expression of L-selectin mRNA and protein in pregnant mice during the periimplantation period and the effect of L-selectin in the embryo implantation. Methods 1. Mice ovigerms were divided into 4 groups： 1-cell stage, 4-cell stage, morula stage and blastocyst stage. The expression of L-selectin mRNA and protein in mice ovigerms was detected by real-time fluorescence quantitative PCR（FQ-PCR） and immunofluorescence histochemistry. The location of L-selectin was analyzed by immunofluorescence histochemistry; 2. Mice were divided into 4 groups： experiment group 1 （1 ： 50）, experiment group 2（ 1 ： 100）, experiment group 3（1：200） and control group （without any intervention to mice comu uteris）. On the morning of day 4 of pregnancy female mice were injected with 5μl of the L-selectin monoclonal antibody into one horn （experiment side） of uteri and with 5μl normal saline into another horn（control side） in the game mouse. The number of embryos implanted was counted and recorded on day 8 of pregnancy to study the effects of the L-selectin on embryo implantation in vivo. The data were analyzed by analysis of variance. Results 1 . With the development and maturation of the ovigerm, the expression of L-selectin mRNA and protein was increased gradually（ P 〈 0.05）, and the level of L-selectin mRNA on blastocyst stage was higher than those of other groups（ P 〈 0.05）. There was a little expression of L-selectin protein in 1-cell stage and 4-cell stage. The expression of L-selectin protein which was mainly distributed around the morula increased significantly. The level of L-selectin protein which was mainly distributed on the cell membrane of trophoblast was the highest during the peri-implantation period（ P 〈 0.05） ; 2. The number of embryos implanted in the horns treated with L-selectin monoclonal antibody was smaller than those in corresponding controls（ P 〈 0.05）. Conclusion L-selectin of the ovigerm might participate in the process o

关 键 词：L-选择素 胚泡 实时荧光定量PCR 免疫荧光组织化学 小鼠 

分 类 号：Q492.6[生物学—生理学]