番荔枝属植物树皮中二萜类成分的HPLC-ELSD分析  

Determination of Ent-Kaurane Diterpenes in Barks from Genus annona by HPLC-ELSD

作　　者：毕志明[1] 蒯玉花[1] 任美婷[1] 李萍[1] 章永红[2] 

机构地区：[1]中国药科大学生药学教研室,南京210038 [2]南京中医药大学第一临床医学院,南京210002

出　　处：《中国药学杂志》2007年第23期1827-1829,共3页Chinese Pharmaceutical Journal

基　　金：江苏省自然科学基金资助项目(BK2002201)

摘　　要：目的建立一种HPLC—ELSD分析方法，测定不同产地、不同种番荔枝属植物树皮中4种贝壳杉烷型二萜的含量。方法采用Agilent ZORBAX 80A Extend—C18柱，0．3％冰醋酸溶液（A）-乙腈（B）梯度洗脱[0～40min，60％～0％（A）；40％-100％（B）]，流速0．6mL·min^-1，蒸发光散射检测器（ELSD）。结果4种贝壳杉烷型二萜与相邻杂质能较好分离，理论塔板数按16α—hydro-19-acetoxy—ent—kauran-17-oic acid（Ⅲ）计算不低于49000。贝壳杉烷型二萜成分以海南产圆滑番荔枝中含量最高。结论该方法精密度高、分离效果好，分析准确，适合于该属植物中二萜类成分的含量测定；不同产地、不同种植物中各成分均存在一定的差别。OBJECTIVE To establish a HPLC-ELSD method for determining the contents of ent-kaurane diterpenes in Genus annona barks from different locations or species. METHODS The separation was performed on a Agilent ZORBAX 80A Extend - C18 column with a gradient elution. The mobile phase consisted of acetic acid and acetonitril with the flow rate at 0.6 mL· min^-1 . The detector was ELSD. RESULTS Four ent-kaurane diterpenes were separated from dopant well. The content of ent-kaurane diterpenes was highest in A. squamosa L. （hainan）. CONCLUSION The method is accurate and suitable for quardity of diterpenes in Genus annona. There is content difference among different locations or species.

关 键 词：HPLC—ELSD 番荔枝属 贝壳杉烷型二萜 

分 类 号：R917[医药卫生—药物分析学]