六种动物含类胰蛋白酶肥大细胞的酶组化分析  

ENZYME HISTOCHEMICAL ANALYSIS OF TRYPTASE CONTAINING MAST CELLS IN SIX SPECIES OF ANIMALS

作　　者：江萍[1] 许乐仁[1] 

机构地区：[1]贵州农学院

出　　处：《畜牧兽医学报》1997年第5期416-421,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金

摘　　要：使用鉴定人类肥大细胞类胰蛋白酶的酶组织化学技术，证实猪、牛、绵羊、狗、猫及小鼠的肥大细胞中均含有类胰蛋白酶。与采用甲苯胺兰的常规组织化学染色比较，类胰蛋白酶阳性肥大细胞与甲苯胺兰阳性肥大细胞在分布、形态和密度方面是相似的。类胰蛋白酶阳性肥大细胞的酶组织化学染色可被利马豆胰蛋白酶抑制物（ＬＢＴＩ）、α－１－抗胰蛋白酶及Ａｐｒｏｔｉｎｉｎ等胰蛋白酶抑制物部分抑制。本研究表明，鉴定人类肥大细胞类胰蛋白酶的酶组织化学技术可以作为鉴定这些动物肥大细胞的一种特异性方法。An enzyme histochemical technique specific for human mast cell tryptase was adopted to stain animal tissues.Mast cells in all tested species including pig,cattle,sheep,dog,cat and mouse were proved to be containing tryptase in their cytoplasmic granules.In comparison with the results of routine histochemical method with toluidine blue,the distribution,morphology and density of the tryptase positive mast cells were similar to those of the toluidine blue positive mast cells.The stainability of the tryptase positive mast cells in these species could be partly inhibited by some trypsin inhibitors such as Lina been trypsin inhibitor(LBTI),α 1 antitrypsin and Aprotinin.The present study showed that the enzymehistochemical technique for human mast cell tryptase could be used as a specific method for the detection of mast cells in these species.

关 键 词：肥大细胞 类胰蛋白酶 酶组织化学 动物 

分 类 号：S852.2[农业科学—基础兽医学]