植物青枯菌的血清分型研究  

THE SEROTYPES OF BACTERIAL WILT PATHOGEN (Pseudomonas solanacearum) IN PLANTS

作　　者：刘琼光[1] 曾宪铭[1] 董春[1] 

机构地区：[1]华南农业大学资源环境学院

出　　处：《华南农业大学学报》1997年第3期33-37,共5页Journal of South China Agricultural University

摘　　要：以代表不同生化型的４个青枯假单胞菌的膜蛋白为免疫原制备了４个相应的抗血清，对我国植物青枯菌的血清分型进行了研究。琼脂双扩散试验结果表明：来自我国６个省（区）的１９种寄主植物的２１２个青枯菌株可分为８个血清型，其中血清型Ⅰ为优势类群，包括了主要茄科植物等１３种寄主的１４７个菌株，占全部供试菌株的６９％。８个血清型代表菌株膜蛋白ＳＤＳ－聚丙烯酰胺凝胶电泳结果表明，它们间存在不同程度上的差异。A study was conducted to determine the serological relationships between strains of Pseudomonas solanacerum in China. Four corresponding antisera were prepared to a kind of membrane protein extracted from cells of four strains representing different biotypes of P. solanacerum respectively. By using Ouchterlong double diffusion tests, 212 isolates from ninteen different hosts in six provinces and cities could be grouped into eight serotypes. It was demonstrated that 147 isolates of them belonged to serotype I which was the predominant one (69%) including thirteen various hosts. SDS PAGE profiles of mebrane proteins extracted from strains representing eight serotypes of P. solanacerum showed that there were some differences between serotypes.

关 键 词：植物 青枯假单胞菌 血清型 

分 类 号：S432.42[农业科学—植物病理学] S432.22[农业科学—农业昆虫与害虫防治]