钆对比剂增强及延迟增强MR扫描监测兔膝关节软骨退变的研究  

An initial experimental study-the value of gadolinium-enhanced MRI and delav enhanced MRI in detecting articular cartilage degeneration of the rabbit knee

作　　者：沈思[1] 刘斯润[1] 朱天缘[1] 梁文彬[1] 黄力[1] 陈汉芳[1] 吴何嘉[1] 

机构地区：[1]广州暨南大学附属第一医院影像中心,510630

出　　处：《中华放射学杂志》2007年第12期1394-1400,共7页Chinese Journal of Radiology

摘　　要：目的探讨钆喷替酸葡甲胺(Gd-DTPA)增强及延迟增强扫描在监测兔膝关节软骨退变的表现及价值。方法选用成年新西兰大白兔20只,按随机数字表法分为 A、B、C、D、E 5组,A、B、C、D 4组分次在右膝关节腔内注入0.2 ml 木瓜蛋白酶(10 U)溶液,为处理侧(A 组1次,B、C、D 组均在1、4、7 d 各1次),建立早期至中晚期的关节软骨退变的动物模型;左膝则注入不含木瓜蛋白酶的稀释液作为对照侧,E 组不作处理,作为空白组对照;A 组于模型制作完成后24 h 行 MR 检查,B 组于第1次注药后24 h 及最后1次注药后24 h 行 MR 检查,C 组于第1次注药后24 h、最后1次注药后24 h 及最后1次注药后1个月行 MR 检查,D 组于第1次注药后24 h、最后1次注药后24 h、最后1次注药后1个月及最后1次注药后3个月行双膝关节矢状面 SE T_1WI、三维抑脂扰相梯度回波(3D-FS-SPGR)序列检查,再通过兔耳缘静脉注入 Gd-DTPA 后,于即刻、2 h、4 h 进行双膝关节同层面、同序列MR 检查,测量软骨的信号强度与腘窝处周围软组织的信号强度的比值(SIR),扫描后即处死动物,取双侧膝关节软骨组织作 HE 染色及检测蛋白多糖含量的阿尔新兰染色,进行观察。结果在3D-FS-SPGR 序列扫描中,Gd-DTPA 增强及延迟增强扫描关节软骨 SIR:A 组增强后即刻扫描,处理侧为1.36±0.20,对照侧为1.14±0.24,增强后2 h 处理侧为1.83±0.21,对照侧为1.39±0.30,增强后4 h 处理侧为1.55±0.32,对照侧为1.42±0.36;B 组增强后即刻扫描,处理侧为1.37±0.23,对照侧为1.05±0.26,增强后2 h 处理侧为1.82±0.22,对照侧为1.24±0.28,增强后4 h 处理侧为1.49±0.25,对照侧为1.27±0.18;C 组增强后即刻扫描,处理侧为1.33±0.27,对照侧为1.07±0.26,增强后2 h 处理侧为2.28±0.20,对照侧为1.29±0.23,增强后4 h 处理侧为2.07±0.51,对照侧为1.48±0.15;D 组增强后即刻扫描,处理侧为1.07±0.38,对照侧为1.40±0.10,增强后2 h 处理侧为1.27±0.09Objective To explore the appearance and value of early and delaye Gadoliniumenhanced MRI in detecting aricular cartilage generation of rabbit knee. Methods Twenty adult New Zealand rabbits were randomly divided into five groups （A, B, C, D, E）. Intra-articular injection of Papain was performed to establish animal models of different stages of cartilage degeneration in the fight knees of A, B, C, D groups, and MR scan was conducted 24 hours,one week,one month and three months after the last Papain injection. The knees were scanned bilaterally with T1WI and 3D-FS-SPGR in sagittal plane. The signal intensity ratio between articular cartilage and surrounding soft tissue was measured at plain scan and 0, 2, 4 hours after intravenous injection of gadolinium （Gd-DTPA）. The articular cartilage was pathologically examined （ HE and alcican blue stain）. Results In 3D-FS-SPGR sequence, it showed significant difference in the SIR between processing side of four groups （F = 7. 961 ,P 〈0.05） and between processing side and comparing side in 0, 2, 4 hours after intravenous injection of Gd-DTPA （ F = 2. 259, P 〈0. 05）. A group （0 h） processing side 1.36 ±0. 20, comparing side 1.14 ±0. 24, （2 h） processing side 1.83 ±0. 21, comparing side 1.39 ±0.30, （4 h） processing side 1.55 ±0. 32, comparing side 1.42 ± 0.36. B group （0 h） processing side 1.37 ± 0. 23, comparing side 1.05 ±0. 26, （2 h） processing side 1.82 ± 0. 22, comparing side 1.24 ± 0. 28, （4 h） processing side 1.49 ± 0. 25, comparing side 1.27 ± 0. 18. C group （0 h） processing side 1.33 ±. 27, comparing side 1.07 ±0. 26, （2 h） processing side 2.28 ± 0. 20, comparing side 1.29 ± 0. 23, （4 h） processing side 2. 07 ± 0. 51, comparing side 1.48 ± 0. 15. D group （0 h） processing side 1.07 ±0. 38, comparing side 1.40 ±0. 10, （2 h） processing side 1.27 ±0.09, comparing side 1.79 ±0. 12, （4 h） processing side 1.41 ±. 19, comparing side 1.89 ± 0. 10. It showed significant difference betw

关 键 词：软骨 关节 软骨疾病 膝关节 磁共振成像 动物实验 

分 类 号：R445.2[医药卫生—影像医学与核医学]