对缺硼反应不同的油菜基因型根系吸收硼特性的研究  

THE RESEARCH ON B ABSORPTION CHARATERISTICS OF TWO RAPE GENOTYPES RESPONDING DIFFERENTLY TO B DEFICIENCY

作　　者：曹享云[1] 刘武定[1] 皮美美[1] 

机构地区：[1]华中农业大学微量元素研究室

出　　处：《华中农业大学学报》1997年第5期335-339,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金;国家教委博士点学科基金

摘　　要：以对缺硼敏感性不同的甘蓝型油菜品种为供试材料，研究其整体根系和离体根吸收硼特性的差异。结果表明，２个品种根系吸硼速率与外界硼浓度呈显著直线相关，直线斜率以敏感品种大于不敏感品种，无论整体根还是离体根都得到同样结果。在不同硼浓度时，２个品种整体根系吸硼速率有明显差异。低硼浓度时（Ｂ０．０５～１．０μｇ／ｇ），敏感品种吸硼速率显著低于不敏感品种；而高硼浓度时（Ｂ＞１．０μｇ／ｇ），则明显高于不敏感品种；采用离体根测定发现，硼进入２个品种根系自由空间过程符合Ｍｉｃｈａｅｌｉｓ－Ｍｅｎｔｅｎ方程，但敏感品种的Ｋｍ值高于不敏感品种，Ｖｍａｘ则小于不敏感品种。不敏感品种在低硼浓度时吸收能力强以及对硼的亲和力高可能是其硼高效的重要原因。This experiment was conducted to study the B absorption charateristics of two typical rape genotypes which had been found to have different susceptibility to B deficiency. For both intact and excised roots of the 2 cultivars, boron absorption rate was linearly correlated with boron concentration in solution, which suggested that boron absorption was a passive, nonmetabolic process. Meanwhile, the slope of the sensitive cultivar (01) was higher than that of the insensitive one (03). The absorption rates by intact roots of the 2 cultivars were apparently different from each other. When the boron concentration was low (B 0.05～1.0 μgg), the absorption rate of the sensitive cultivar was much lower than that of the insensitive one, but at a higher boron concentration (>1.0 μgg) the former was much higher. The process of boron entering into the free space could be described by MichaelisMenten equation. The dynamic parameters ( V max, K m) indicates that boron influx into the free space of the insensitive cultivar faster than that of the sensitive one and in langer quantity.

关 键 词：缺硼 油菜 基因型 硼吸收 硼肥 

分 类 号：S565.401[农业科学—作物学]