金线莲人工种子制作技术及萌发研究  

Studies on Preparation Technique and Germination of Anoectochilus roxburghii Synthetic Seeds

作　　者：张明生[1] 李花[1] 阚世超[1] 张丽霞[1] 

机构地区：[1]贵州大学生命科学学院

出　　处：《种子》2007年第11期50-53,共4页Seed

基　　金：贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2005]350号);贵州大学引进人才科研项目(X060044)资助

摘　　要：以金线莲为实验材料,通过液体悬浮培养建立原球茎悬浮系,并以原球茎为人工种子的繁殖体,研究了人工种皮基质、人工胚乳组分、贮藏条件、萌发基质等对人工种子萌发率和成苗率的影响。结果表明,以4%海藻酸钠+2%CaCl2+2%壳聚糖为人工种皮基质,1/2MS液体培养基+0.2mg/LNAA+0.1mg/LGA3+0.5mg/LBA+0.4mg/L青霉素+0.3%多菌灵粉剂+0.2%苯甲酸钠+1.0%蔗糖+1.0%活性炭作为人工胚乳成分制作的人工种子,其萌发率和成苗率最高。这样的人工种子在4℃条件下贮藏20天后的萌发率及成苗率分别为58.3%和55.5%,随着贮藏温度的升高和贮藏时间的延长,人工种子的萌发率和成苗率均大幅度下降。The protocorm-suspension-system ofAnoectochilus roxburghii （Wall.）Lindl was founded by liquidsuspension culture. The factors to affect germination rate and seedling conversion rate of Anoectochilus roxburghii synthetic seeds, such as synthetic coating materials, synthetic endosperm components,storing conditions and germination materials, etc. were studied. The results showed that the germination rate and seedling conversion rate of synthetic seeds were the highest on the MS solid-medium while using 4% sodium alginate ＋ 2% CaCl2 ＋ 2% chitosan as synthetic coating materials,with 1/2 MS liquid-medium ＋ 0.2 mg/L NAA ＋ 0.1 mg/L GA3 ＋0.5 mg/L BA ＋ 0.4 mg/L penicillin ＋ 0.3% carbendazim powder ＋ 0.2% sodium benzoate ＋ 1.0% sucrose ＋ 1.0% active carbon as synthetic endosperm. And the germination rate and seedling conversion rate of synthetic seeds could attain to 58.3% and 55.5% after 20 days storing at 4 ℃. The germination rate and seedling conversion rate of synthetic seeds decreased to a great extent with increasing the storing temperature and prolonging storing time.

关 键 词：金线莲 人工种子 萌发率 成苗率 

分 类 号：S682.31[农业科学—观赏园艺]