弯曲菌菌种冷冻保存及复苏方法的研究  

Studies of Methods of Freezing Storage of Campylobacter and Recovery of Frozen Storage Strain

作　　者：骆海朋[1] 陈倩[1] 

机构地区：[1]北京市疾病预防控制中心,北京100013

出　　处：《中国食品卫生杂志》2007年第6期518-519,共2页Chinese Journal of Food Hygiene

摘　　要：目的探索一种简单、方便、有效的弯曲菌菌种保存方法。方法采用含30%甘油的脑心浸液肉汤,将实验室保存的19株弯曲菌于-30℃和-70℃分别保存,每个菌株3个菌种管。1年后,采用5%脱纤维羊血的布氏肉汤进行增菌,然后转种哥伦比亚血平板,比较存活效果。结果-30℃条件下采用多管法保存弯曲菌可以获得与-70℃条件下保存相当的效果。结论采用多管法(每株菌3个菌种管)于-30℃冷冻保存弯曲菌是一个简单、方便、有效、实用的方法。Objective To find an easy and effective method for the, storage of campylobacter. Method Campylobacter strains were stored in the content of 30% glycerine brain heart broth. 19 isolates were used in this study and stored at - 30 ℃ and -70 ℃ and every strain had 3 copies. After one-year store, the frozen strains were recovered in the content of 5% sheep blood preston broth for 24 hours, and then were subcuhured on the Columbia blood plate. Results In one-year store, the storage of the strains in the temperature of - 30℃ had the same effect as the storage of strains in the temperature of - 70 ℃ . Conclusion The method of storage of campylobacter in muhi tubes in the temperature of - 30 ℃ may be an easy and effective method .

关 键 词：弯曲杆菌属 保存 生物学 低温保存 

分 类 号：R117[医药卫生—公共卫生与预防医学]