罗布麻叶抗抑郁活性部位的筛选  

Screening of Antidepressant Fractions of the Leaves of Apocynum Venetum L.

作　　者：周本宏[1] 冯琪[2] 李小军[3] 王慧媛[2] 刘敏[2] 黄付伟[2] 王高华[1] 胡先明[2] 

机构地区：[1]武汉大学人民医院 [2]武汉大学药学院 [3]武汉大学基础药理学系

出　　处：《中国药师》2007年第12期1173-1175,共3页China Pharmacist

摘　　要：目的:筛选罗布麻叶的抗抑郁活性部位,并探讨抗抑郁活性与总黄酮含量的关系。方法:用小鼠强迫游泳实验评价抗抑郁作用;用荧光分光光度法测定单胺类神经递质;用紫外分光光度法测定总黄酮的含量。结果:罗布麻叶乙醇提物对小鼠的不动时间没有显著的影响,而其他4个部位均可不同程度的降低小鼠的不动时间,其中正丁醇部位和水相部位的作用最强。罗布麻叶乙醇提取物显著降低了5-HT在纹状体中的浓度,而石油醚、乙酸乙酯、正丁醇和水相部位对NA、DA、5-HT在海马,纹状体中的浓度有不同程度的影响。结论:罗布麻叶醇提物没有抗抑郁活性,但其他四个部位具有抗抑郁活性,其活性成分主要分布在正丁醇和水溶性部位,且抗抑郁活性与总黄酮含量没有线性关系。Objective： To screen the antidepressant fractions of the leaves of Apocyunm venetum L. （AV）. And to study the relationship between the antidepressant effect and the content of flavonoids. Method： Forced swimming test was used to evaluate antidepressant activity. The levels of monoamine neurotransmitters were determined with spectrofluorophotometer and the content of flavonoids was determined with ultraviolet spectrophotometry. Result： The effect of ethanolic extracts of AV on immobility time was not marked, but its other four fractions respectively reduced the duration of immobility. N-butanol fraction and water soluble fraction were more potent than that any other test sample. Ethanolic extracts of AV significantly reduced the level of 5-HT in striatum. And petrol fraction, ethyl acetate fraction, n-butanol fraction and water soluble fraction had different effects on the levels of the NA, DA and 5-HT in hippocampus and striatum. Conclusion： Ethanolic extracts of AV has no antidepressant activity while its other four fractions do. There isn＇ t linear relationship between the antidepressant effect and the content of flavonoids.

关 键 词：罗布麻叶 抗抑郁 强迫游泳实验 单胺类神经递质 黄酮 

分 类 号：R285.5[医药卫生—中药学]