化学发光法测定氨苄西林钠  

Chemiluminescence determination of ampicillin sodium

作　　者：石文兵[1] 杨季冬[1] 张君[1] 

机构地区：[1]涪陵师范学院化学及环境科学系,涪陵408003

出　　处：《分析试验室》2007年第12期60-63,共4页Chinese Journal of Analysis Laboratory

基　　金：重庆市教委资助(KJ061306);重庆市科技攻关计划资助(2006AA7003);发光与实时分析重庆市重点实验室开放基金项目资助

摘　　要：在NaOH介质中,KIO4氧化鲁米诺产生化学发光,氨苄西林钠显著增强该体系的发光。据此,建立了一种简单、快速测定氨苄西林钠的流动注射化学发光新方法。在优化的实验条件下,线性范围为0.01-10μg/mL,检出限为3.0 ng/mL,对1.0μg/mL氨苄西林钠进行了11次平行测定,其相对标准偏差为1.4%。已用于粉针剂、合成样品及尿样中氨苄西林钠的测定。It was found that the chemiluminescence produced by the oxidation of luminol by postassium periodate in the basic medium was enhanced by ampicillin sodium. A new method has been developed for the determination of ampicillin sodium. Under the optimum conditions, CL intensities are proportional to the concentrations of ampicillin sodium in the range of 0.01 - 10 μg/mL. The limit of detection is 3.0 ng/mL for ampicillin sodium and the relative standard deviation （RSD） is 1.4 % for 1.0 μg/mL ampicillin sodium （n = 11）. The method has been applied to the determination of ampicillin sodium in the injections, synthetical samples and urine samples with satisfactory results.

关 键 词：化学发光 流动注射分析 氨苄西林钠 尿样 

分 类 号：O557.3[理学—热学与物质分子运动论]