离子交换柱后衍生流动注射分光光度在线连续测定水中NO_2^-和NO_3^-  

On-line simultaneous determination of nitrite and nitrate in water samples by flow injection spectrophotometry in combination with post-column derivatization

作　　者：吴宏[1] 张立新[1] 王锦化[1] 赵长春[1] 

机构地区：[1]徐州师范大学化学系,徐州221116

出　　处：《分析试验室》2007年第12期87-90,共4页Chinese Journal of Analysis Laboratory

基　　金：徐州师范大学科研基金(03XLB13);江苏省高校自然科学基础研究(07KJB150112)项目资助

摘　　要：建立了连续测定NO2-和NO3-的柱后在线衍生结合流动注射光度分析体系。阴离子交换柱(HPIC-AS3)分离水样中的NO2-和NO3-,洗脱液依次将NO2-和NO3-洗脱流经镀铜镉还原柱,NO3-在线还原为NO2-,与对氨基苯磺酸溶液和N-(1-萘基)-乙二胺溶液合并,在λmax=540 nm处对NO2-和NO3-产生的红色染料进行光度连续检测。NO2-和NO3-的线性范围分别为0.01~1.0mg/L和0.02~2.0 mg/L,检出限分别为0.004和0.008 mg/L。方法用于雨水、湖水和自来水中痕量NO2-和NO3-的同时连续测定。The on-line post-column derivatization system coupled to flow injection spectrophotometry was developed for the simultaneous determination of trace nitrite and nitrate. After preconcentration and separation on an anion-exchange column （HPIC-AS3）, nitrite and nitrate were introduced to a copper-coated cadmium reactor, in which nitrate was reduced to nitrite, then merged with p-aminobenzenesulfonic acid solution and N-（ 1-Naphthyl）ethylenediamine dihydrochloride solution, respectively. The red dye successively formed by the reactions derived from nitrite and nitrate were spectrophotometrically detected at λmax= 540 nm. The linear range for the determination of nitrite and nitrate are 0.01 to 1.0 and 0.02 to 2.0 mg/L with detection limits of 0. 004 and 0. 008 mg/L, respectively. This method has been successfully applied to the determination of trace nitrite and nitrate in several real water samples.

关 键 词：柱后衍生 流动注射 分光光度法 NO2^- NO3^- 

分 类 号：O557.3[理学—热学与物质分子运动论]