转Bar基因抗除草剂两系杂交早稻恢复系Bar 68-1的培育研究  

Studies on Development of Bar 68-1,a Bar-transgenic Restorer Line with Herbicide Resistance of Two-line Early Hybrid Rice

作　　者：肖国樱[1] 唐俐[2] 袁定阳[2] 邓晓湘[2] 袁隆平[2] 辛世文[3] 

机构地区：[1]中国科学院亚热带农业生态研究所,湖南长沙410125 [2]国家杂交水稻工程技术研究中心,湖南长沙410125 [3]香港中文大学生物系

出　　处：《杂交水稻》2007年第6期57-61,共5页Hybrid Rice

基　　金：香港AOE项目(AOE/B-07/99);中国科学院知识创新工程重要方向项目(KZCX3-SW-434);湖南省重大科技专项(超级杂交水稻技术研究与示范)

摘　　要：通过基因枪转化方法将抗除草剂基因Bar转入早籼稻两系恢复系D68中,育成了转基因抗除草剂恢复系Bar 68-1和杂交早稻组合香125S/Bar 68-1;Southern杂交检测和除草剂抗性鉴定结果显示,它们具有抗除草剂特性并能稳定遗传。转基因抗除草剂杂交稻组合香125S/Bar 68-1和对照香两优68(香125S/D68)相比,株高显著降低,直链淀粉含量极显著降低,其它农艺、品质和产量性状没有明显变化。A herbicide resistant gene Bar was transferred into a two-line indica restorer line D68 by panicle bombardment,and the developed transgenic restorer line Bar 68 -1 and its hybrid Xiang 125S/Bar 68 -1 showed herbicide resistance and stable inheritance based on the Southern blotting analysis and the identification of herbicide resistance. When the transgenic hybrid rice Xiang 125S/Bar 68 - 1 was compared with the check hybrid rice Xiang 125S/D68 ,the plant height and amylose content of the transgenic hybrid rice were decreased significantly at 5% and 1% levels, respectively, but all of the other major agronomic and yield characters were not statistically different from each other.

关 键 词：两系杂交水稻 转BAR基因水稻 抗除草剂恢复系 BAR 68—1 选育 

分 类 号：S511.03[农业科学—作物学] Q78[生物学—分子生物学]