银屑病患者皮损p16^INK4a基因CpG甲基化位点和频度  

The methylation locus and frequency pattern on p16^INK4a gene promoter CpG in epidermis of patients with psoriasis

作　　者：陈敏[1] 崔盘根[1] 姚煦[1] 曹元华[1] 弓娟琴[1] 李安生[2] 陈志强[1] 

机构地区：[1]中国医学科学院中国协和医科大学皮肤病研究所皮肤病研究室,南京210042 [2]中国医学科学院中国协和医科大学皮肤病研究检验科,南京210042

出　　处：《中华医学遗传学杂志》2007年第6期674-676,共3页Chinese Journal of Medical Genetics

基　　金：江苏省人事厅“六大人才高峰”项目;国家自然科学基金（30170863）

摘　　要：目的研究皮损表皮p16^INK4a甲基化阳性的银屑病患者基因启动子区CpG甲基化的位点和频度。方法采集50例银屑病患者皮损处皮肤,分离表皮,提取DNA。采用甲基化特异性PCR和测序法检测p16^INK4a基因启动子区CpG甲基化的位点和频度。结果p16^INK4a基因启动子甲基化阳性的患者,约50%CpG发生甲基化,均出现于特定位点。结论p16^INK4a甲基化阳性银屑病患者p16INK4a基因启动子并非完全发生甲基化。Objective To investigate the CpG methylation locus and frequency pattern on p16^INK4a gene promoter in epidermis of p16^INK4amethylated patients with psoriasis vulgaris. Methods The DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients. Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylafion in p16^INK4a gene promoter region. Results Approximately 50% CpG was methylated in p16^INK4a methylated patients, methylation was found in specifical locus of p16^INK4a gene promoter. Conclusion The distinct methylation pattern is showed on the p16^INK4a gene promoter region in patients with psoriasis.

关 键 词：银屑病 P16^INK4A基因 CpG甲基化 启动子 

分 类 号：R686[医药卫生—骨科学]