姜黄素对单侧输尿管梗阻大鼠肾小管上皮细胞转分化影响的研究  

Effect of curcumin(姜黄素) on trans-differentiation of renal tubular epithelial cells in rats with unilateral ureteral obstruction

作　　者：赵爱青[1] 张晓明[1] 侯恒[1] 李荣山[2] 马存根[1] 

机构地区：[1]山西大同大学医学院内科教研室,山西大同037008 [2]山西医科大学第二临床医学院肾内科,山西太原030001

出　　处：《中国中西医结合急救杂志》2007年第6期348-351,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：山西省自然科学基金资助项目(20021120)

摘　　要：目的:探讨姜黄素对单侧输尿管梗阻(UUO)大鼠肾小管上皮细胞转分化的作用。方法:采用UUO致肾间质纤维化大鼠模型。将24只大鼠随机均分为假手术组、模型组和姜黄素组。从制模后2 d起,姜黄素组给予100 mg.kg-1.d-1姜黄素腹腔注射。术后4周心脏穿刺抽血,测定各组大鼠血清尿素氮(BUN)和血肌酐(SCr)值。处死大鼠,用苏木素-伊红(HE)、Masson染色评定肾小管间质纤维化程度;用免疫组化方法检测肾组织内转化生长因子-β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)、波形蛋白(Vimentin)的蛋白表达部位及表达水平。结果:与假手术组比较,模型组大鼠BUN水平显著增加(P<0.01),肾间质纤维组织相对面积显著扩大(P<0.01),肾组织内TGF-β1、α-SMA和Vimentin的蛋白表达均显著上调(P均<0.01)。姜黄素干预后,上述上调指标都被显著抑制(P<0.05或P<0.01)。结论:姜黄素可能通过抑制UUO大鼠肾小管上皮细胞转分化而减轻肾间质纤维化。Objective： To investigate the effect of curcumin（姜黄素） on trans-differentiation of renal tubular epithelial cells in rats with unilateral ureteral obstruction（UUO）.Methods： UUO rat animal models were used.Twenty four rats were randomly divided into three groups（each n=8）： sham operation group（0.9% saline）,model group（dimethyl sulphoxide）,and curcumin treatment group.Two days after the establishment of the model,curcumin 100 mg·kg^-1·d^-1 was given intra-peritoneally in curcumin treatment group.All rats were killed 4 weeks after surgery.Firstly,the blood obtained from heart puncture was used for the examination of blood urea nitrogen（BUN） and serum creatinine（SCr） in each rat.Secondly,the degree of tubulointerstitial fibrosis was scored by hematoxylin and eosin（HE） and Masson staining,and the sites and levels of protein expression of transforming growth factors,type beta1（TGF-β1）, α-smooth muscle antibody（α-SMA） and Vimentin were detected by immunohistochemistry staining.Results： Compared with shame-operation group,the BUN level was significantly increased（P〈0.01）,and the relative area of interstitial fibrosis was also significantly enlarged in the model group（P〈0.01）.Compared with shame-operation group,the expression of TGF-β1,α-SMA and Vimentin protein was significantly up-regulated in their kidney tissue（all P〈0.01）.After intervention with curcumin, the upregulation of the above-mentioned parameters was significantly inhibited（P〈0.05 or P〈0.01）.Conclusion： The ameliorating effect of curcumin on renal interstitial fibrosis may be partly mediated by inhibiting the trans-differentiation of renal tubular epithelial cells towards myofibroblast in rats with UUO.

关 键 词：姜黄素 Α-平滑肌肌动蛋白 波形蛋白 肾小管上皮细胞转分化 

分 类 号：R285.5[医药卫生—中药学] R693.2[医药卫生—中医学]