生脉注射液对兔肺缺血/再灌注损伤时细胞凋亡的影响  

Effect of Shengmai injection(生脉注射液) on pneumocyte apoptosis during pulmonary ischemia/reperfusion injury in rabbits

作　　者：林丽娜[1] 张圣恭[1] 王万铁[2] 邱晓晓 戴雍月[2] 王青[2] 

机构地区：[1]温州医学院附属第一医院麻醉科,浙江温州325000 [2]温州医学院病理生理学教研室,浙江温州325035

出　　处：《中国中西医结合急救杂志》2007年第6期361-363,I0011,共4页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：浙江省中医药管理局科研基金(2005C088);浙江省温州市科技计划重点项(Y2005A080)

摘　　要：目的:观察生脉注射液(SMI)对兔肺缺血/再灌注(I/R)损伤中细胞凋亡的影响。方法:将30只日本大耳白兔按随机数字表法分为3组,每组10只。用开胸后阻断左肺门60 min、再灌注180 min制备兔单侧肺I/R损伤模型。对照组仅行开胸,于左肺门过阻断带并观察240 min。SMI组于缺血前20 min经耳缘静脉注射SMI 15 ml/kg,其余操作同I/R组。于再灌注180 min时静脉注入氯化钾处死动物,取各组肺组织,观察肺湿/干重(W/D)比值、超氧化物歧化酶(SOD)活性、丙二醛(MDA)浓度、一氧化氮(NO)含量、肺损伤定量评价指标(IQA)及肺组织细胞凋亡指数(AI)。结果:与对照组比较,I/R组肺组织SOD活性、NO含量均明显降低(P均<0.01),MDA、W/D比值、IQA、AI均明显升高(P均<0.01)。与I/R组比较,SMI组SOD活性、NO含量均明显升高(P<0.01和P<0.05),MDA、W/D比值、IQA、AI均不同程度降低(P均<0.01)。AI与MDA、W/D比值、IQA均呈显著正相关(r1=0.835,r2=0.751,r3=0.656,P均<0.01),与SOD、NO呈显著负相关(r4=-0.769,r5=-0.488,P<0.01和P<0.05)。结论:SMI可通过提高体内NO水平、降低氧自由基水平、减轻脂质过氧化反应、抑制肺组织细胞凋亡,从而达到减轻肺损伤的作用。Objective： To investigate the effect of Shengmai injection（SMI,生脉注射液） on pneumocyte apoptosis during pulmonary ischemia/reperfusion（I/R） injury in rabbits.Methods： Thirty rabbits used for unilateral lung I/R model were randomly divided into three groups（each n=10）： control group or the sham operation group（C group with observation for 240 minutes）,I/R group and SMI group（15 ml/kg SMI intravenously injected 20 minutes before ischemia）.The model of rabbit lung I/R injury was replicated by ischemia for 60 minutes and reperfusion for 180 minutes.Rabbits were sacrificed after injected KCl at 180 minutes after reperfusion and lung tissues were harvested.The activity of superoxide dismutase（SOD）,the content of plasma nitric oxide（NO）,the concentration of malondialdehyde（MDA）,the wet to dry weight ratio of lung tissue（W/D）,the index of quantitative assessment of histologic lung injury（IQA） and apoptosis index（AI） at 180 minutes after reperfusion in the lung tissues were measured respectively in different groups.Results： The activity of SOD and the content of NO were significantly lower in I/R group than in C group（both P〈0.01）;while the content of MDA,the value of W/D,IQA,AI were evidently higher in I/R group than in C group（all P〈0.01）.SMI group in contrast with I/R group showed that the levels of SOD and NO were higher（P〈0.01 and P〈0.05）,however,the value of MDA,W/D,IQA,and AI were lower in different degrees（all P〈0.01）.AI was positive correlated with MDA,W/D,IQA（r1=0.835,r2=0.751,r3=0.656,all P〈0.01）,while was negative correlated with SOD,NO（r4=-0.769,r5=-0.488,P〈0.01 and P〈0.05）.Conclusion： SMI can suppress apoptosis of lung tissue cell during pulmonary I/R injury,which results in the obvious attenuation of the injury of pneumocyte by raising NO level,dropping oxygen free radicals level and decreasing lipid peroxidation.

关 键 词：缺血/再灌注损伤 肺 氧自由基 一氧化氮 凋亡 生脉注射液 

分 类 号：R285.5[医药卫生—中药学] R256.1[医药卫生—中医学]