丙肝病毒非结构蛋白基因NS5A的克隆化  

Cloning and identification of nonstructural protein5A of hepatitis C virus

作　　者：陈立冬[1] 张连峰[1] 成军[2] 洪源[2] 韩莉[1] 

机构地区：[1]郑州大学第一附属医院消化内科,郑州450052 [2]北京地坛医院传染病研究所

出　　处：《临床医学》2007年第9期76-77,共2页Clinical Medicine

摘　　要：目的克隆化非结构蛋白基因NS5A。方法根据NCBI的Genbank上公布的HCV-1b型基因序列,设计PCR产物为NS5A的特异性引物,以HCV-1b型RNA为模板,利用RT-PCR方法扩增出1341 bp片段,连接至pGEM-T载体并送测序,测序结果表明NS5A已克隆成功。结果成功克隆了丙肝病毒非结构蛋白基因NS5A。结论NS5A是一种典型的病毒基因组编码的多功能蛋白,NS5A的克隆化为进一步研究NS5A的分子生物学机制和探索新型治疗技术奠定基础。Objective To screen and clone hepatitis C virus（HCV） nonstructural protein 5A（NS5A）.Methods The specific primers were designed based on the HCV-1b sequence published by NCBI,and the PCR products were NS5A sequence.A 1341 bp DNA fragment was extracted and amplified by reverse transcription polymerase chain reaction（RT-PCR）,and ligated into pGEM-T cloning vector.The recombinant pGEM-T vector was sent to be sequenced.After sequence,the NS5A sequence was cloned successfully.Results NS5A was successfully cloned.Conclusison NS5A is a multifunction protein.These results will pave the way for the study of the molecular mechanism of NS5A protein of HCV and the development of new therapy for chronic hepatitis C.

关 键 词：丙型肝炎病毒 非结构蛋白5A 反转录-聚合酶链反应 

分 类 号：R512.63[医药卫生—内科学]