HIV／HCV合并感染者淋巴细胞活化及第二受体表达的研究  

The frequency of lymphocytes and the activation, coreceptor expression in HIV/HCV co-infected patients of China

作　　者：施万英[1] 张子宁[1] 姜拥军[1] 李革飞[1] 金鑫[1] 王亚男[1] 张旻[1] 韩晓旭[1] 代娣[1] 刘静[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院,卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2007年第11期1026-1031,共6页Chinese Journal of Microbiology and Immunology

基　　金：卫生部艾滋病防治应用性研究项目（WA2006-2）;国家“十五”科技攻关课题（2004BA719A12）;辽宁省优秀青年科研人才培养资金资助项目（3040011）

摘　　要：目的了解中国不同疾病进展阶段人类免疫缺陷病毒和丙型肝炎病毒（HIV／HCV）合并感染者T淋巴细胞与自然杀伤细胞（natural killer cells，NK）数量变化及T淋巴细胞活化、受体表达情况，并探讨HCV感染对HIV感染免疫指标及疾病进展的影响。方法应用流式细胞术分析228例不同疾病进展阶段的HIV／HCV合并感染者及101例单纯HIV感染者外周血T淋巴细胞、NK细胞数量及T淋巴细胞活化受体（HLA-DR、CD38）、第二受体（CCR5、CXCR4）表达情况。结果（1）HIV／HCV合并感染组中，CD4^＋T淋巴细胞、NK细胞数量随疾病进展持续下降，其中艾滋病组（AIDS）明显低于无症状HIV感染组（HIV）（P〈0．05），HIV组明显低于长期不进展组（LTNP）（P〈0．01），LTNP组与健康对照组差异无统计学意义。LTNP组、HIV组及AIDS组CD4^＋、CD8^＋T细胞表面活化受体HLA-DR、CD38的表达依次升高，其中各组间CD8／CD38的升高差异均有统计学意义（P〈0．05），AIDS组CD4／HLA-DR、CD8／HLA-DR的升高明显高于LTNP组和HIV组（P〈0．01）。LTNP组、HIV组及AIDS组CD4^＋、CD3^＋T细胞表面CCR5的表达亦依次升高，各组间差异均有统计学意义（P〈0．05）；CD3^＋T细胞表面CXCR4的表达依次升高，AIDS组明显高于HIV组和LTNP组（P〈0．01）。（2）HIV／HCV合并感染组与单纯HIV感染组相比，AIDS组NK细胞明显下降（P〈0．05），CD4^＋T细胞下降，但无统计学意义，CD4／HLA-DR、CD8／HLA-DR、CD4／CXCR4、CD3／CXCR4明显升高（P〈0．01）；HIV组NK细胞明显下降（P〈0．01），CD4／CXCR4明显升高（P〈0．05）；LTNP组各项指标与单纯HIV感染组相比差异无统计学意义。（3）HIV／HCV合并感染组的HIV病毒载量随疾病进展不断升高，与单纯HIV感染组相比差异无统计学意义；HCV病毒载量在疾病不同阶段差异无统计学意义（P〉0．05）。结论随疾病进展，HIV／HCV合并�Objective To characterize the frequencies of T lymphocytes, natural killer（NK） cells, and activation, coreceptor expression of T lymphocytes of Chinese HIV/HCV co-infected patients, and to study the im-pact of HCV on the immune status and disease progression of HIV infection. Methods Cross-sectional compari-sons were made among 228 HIV/HCV co-infected patients and 101 HW mono-infected patients. A flow cytometry was used to analyze the numbers of T lymphocytes, NK cells and the level of activating markers（HLA-DR and CD38） and coreceptors（CCR5 and CXCR4） of T lymphocytes in different disease stages. Results （1） Along with the progression of HIV/HCV co-infection, patients tended to have lower numbers of CD4^＋ T cells and NK cells, with significant differences among groups （P〈0.05）. Meanwhile, the activation levels of T lymphocytes in the long-term nonprogressors （LTNP） group, the HW group and the AIDS group increased gradually. The levels of CDS/CD38 are significantly different among groups（P〈0.05）, and the levels of CD4/HLA-DR, CDS/HLA-DR in AIDS are considerably higher than those of the HIV group and the LTNP group（P〈0.01）. The expression of CCR5 on CD4^＋ and CD3^＋ T cells increased significantly by the order of the LTNP group, the HIV group and the AIDS group（P〈0.05）. The level of CXCR4 on the CD3^＋ T cell in the AIDS group is significantly higher than that of the HIV-infected group and the LTNP group（P〈0.01）. （2） Compared with HIV mono-infected patients, the counts of NK cells of HIV/HCV co-infected patients decreased significantly in the HIV-infected group and the AIDS group （P〈 0.05）, and the levels of CD4/HLA-DR, CD8/HIA-DR, CD4/CXCR4 and CD3/CXCR4 in-creased significantly in the AIDS group（P〈0.01）. （3） The viral load of HIV and HCV in HIV/HCV co-infected group increased gradually with the progression of disease, and there are no significant differences compared with HIV mono-infecfion. Conclusion Mong with the progression of HIV/HC

关 键 词：人类免疫缺陷病毒 丙型肝炎病毒 自然杀伤细胞 第二受体 活化 

分 类 号：R686[医药卫生—骨科学]