补肾醒脑方对实验性血管性痴呆大鼠脑神经细胞凋亡的影响  

Effects of Bushenxingnao Prescription on Cerebral Neurocyte Apoptosis of Experimental Vascular Dementia Rats

作　　者：郑攀[1] 封银曼[1] 郑宏[2] 杨新年[1] 夏寒星[1] 

机构地区：[1]河南中医学院,河南郑州450008 [2]河南中医学院第一附属医院,河南郑州450008

出　　处：《中国中医药信息杂志》2007年第12期19-20,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河南省杰出青年科学基金资助(0112001300)

摘　　要：目的观察补肾醒脑方对血管性痴呆(VD)大鼠脑神经细胞凋亡的影响,探讨其病理机制。方法采用反复夹闭双侧颈总动脉伴低血压造成拟VD大鼠模型,通过原位末端标记(TUNEL)法检测脑神经细胞凋亡情况。结果补肾醒脑高、低剂量组可抑制缺血再灌注后神经细胞的凋亡。结论补肾醒脑方能抑制脑神经细胞凋亡,缓解兴奋性毒性损伤,保护大脑神经细胞,这可能是补肾醒脑方治疗VD的作用机制之一。Objective To study the pathology mechanism of vascular dementia （VD） rats and study the effect of Bushenxingnao （BSXN） prescription on cerebral neurocyte apoptosis. Method Experimental VD model rats were caused by clipping two side of total artery in neck repeatedly and to examine cerebral neurocyte apoptosis with TUNEL. Results Both the high and low dose BSXN abated excitatory toxic infury and inhibit neurocyte apoptosis after ischemia-refusion. Conclusion BSXN prescription can repress cerebral neurocyte apoptosis, to abate its excitatory nerotoxicity, to protect cerebral neurocyte, which may be one of the mechanisms of to treating the lesion.

关 键 词：补肾醒脑方 血管性痴呆 原位末端标记 细胞凋亡 大鼠 

分 类 号：R289.11[医药卫生—方剂学]