Immobilization of RGD Peptidcs onto Decellularized Valve Scaffolds to Promote Cell Adhesion  

作　　者：史嘉玮 

机构地区：[1]Department of Cardiovascular Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2007年第4期686-690,共5页武汉理工大学学报（材料科学英文版）

基　　金：the National Natural Science Foundation of China(No.30371414,30571839,30600608)

摘　　要：Porcine aortic valves were decellularized with trypsinase/EDTA and Triton-100. With the help of a coupling reagent Sulfo-LC-SPDP, the biological valve scaffolds were immobilized with one of RGD （arginine-glycine-aspartic acid） containing peptides, called GRGDSPC peptide. Myofibroblasts harvested from rats were seeded onto them. Based on the spectra of X-ray photoelectron spectroscopy, we could find conjugation of GRGDSPC peptide and the scaffolds. Cell count by both microscopy and MTT assay showed that myofibroblasts were easier to adhere to the modified scaffolds. It is proved that it is feasible to immobilize RGD peptides onto decellularized valve scaffolds, and effective to promote cell adhesion, which is beneficial for constructing tissue engineering heart valves in vitro.Porcine aortic valves were decellularized with trypsinase/EDTA and Triton-100. With the help of a coupling reagent Sulfo-LC-SPDP, the biological valve scaffolds were immobilized with one of RGD （arginine-glycine-aspartic acid） containing peptides, called GRGDSPC peptide. Myofibroblasts harvested from rats were seeded onto them. Based on the spectra of X-ray photoelectron spectroscopy, we could find conjugation of GRGDSPC peptide and the scaffolds. Cell count by both microscopy and MTT assay showed that myofibroblasts were easier to adhere to the modified scaffolds. It is proved that it is feasible to immobilize RGD peptides onto decellularized valve scaffolds, and effective to promote cell adhesion, which is beneficial for constructing tissue engineering heart valves in vitro.

关 键 词：RGD peptide decellularized valve scaffold cell adhesion tissue engineering heart valve 

分 类 号：R318[医药卫生—生物医学工程]