桦木尘和柞木尘对大鼠肺泡巨噬细胞分泌细胞因子的影响  

Effect of birch and oak dusts on the cytokines secreted from alveolar macrophages in rats

作　　者：杨海贤[1] 白景文[1] 赵钢[1] 曾嘉[1] 

机构地区：[1]天津医科大学电镜测试中心

出　　处：《中华劳动卫生职业病杂志》1997年第4期207-210,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：为研究体外桦木尘（ＢＤ）和柞木尘（ＯＤ）对大鼠肺泡巨噬细胞（ＡＭ）分泌细胞因子的影响，从支气管肺泡灌洗液中分离３０只Ｗｉｓｔａｒ大鼠的ＡＭ，进行体外培养，分别以不同剂量的ＢＤ或ＯＤ（１００、１５０、２００、２５０μｇ／ｍｌ）诱导ＡＭ，于不同时间（４、２４、４８、７２小时），检测ＡＭ分泌ＩＬ－１、ＩＬ－２、ＩＬ－６和ＴＮＦα的活性。结果显示：ＢＤ和ＯＤ诱导ＡＭ分泌ＩＬ－１、ＩＬ－２、ＩＬ－６和ＴＮＦα，其活性均高于或明显高于对照组（Ｐ＜０．０５或Ｐ＜０．０１），而ＢＤ组与ＯＤ组相比，也有不同或明显不同（Ｐ＜０．０５或Ｐ＜０．０１）。在２００μｇ／ｍｌ的时间曲线中也显示ＢＤ组高于或明显高于ＯＤ组（Ｐ＜０．０５或Ｐ＜０．０１）。提示：ＢＤ和ＯＤ均能刺激ＡＭ分泌ＩＬ－１、ＩＬ－２、ＩＬ－６和ＴＮＦα的活性增加，而ＢＤ较ＯＤ更为明显。The study was to find out the effect of birch and oak dusts on the cytokines secreted from rat alveolar macrophages(AM) in vitro.Alveolar macrophages were harvested from 30 rats by tracheal lavage.The cells were incubated separately with different doses(100,150,200,250 μg/ml) of birch (BD) or oak(OD) dusts,and the cultures were incubated for different time(4,24,48 and 72 h). The activities of interleukin 1(IL 1),interleukin 2(IL 2),interleukin 6(IL 6)and tumor necrosis factor α (TNFα) were determined separately.The results revealed that:active secretion of IL 1,IL 2,IL 6 and TNFα was induced in AM by BD or OD,resuling in higher or obviously higher activity of the cytokines than that in controls.Varying degrees of difference also existed between BD and OD groups( P <0.05 or P <0.01),ie more obvious effect of BD than that of OD.

关 键 词：肺泡 巨噬细胞 桦木尘 细胞因子 粉尘 

分 类 号：R135.2[医药卫生—劳动卫生] R361.3[医药卫生—公共卫生与预防医学]