Calcofluor White M2R荧光染色法识别家蚕微孢子虫  

Staining and discrimination of Nosema bombycis spores with Calcofluor White M2R

作　　者：刘吉平[1] 曾玲[2] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]华南农业大学昆虫生态研究室,广州510642

出　　处：《昆虫学报》2007年第11期1185-1186,共2页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30671588);广东省科技攻关项目(2004B50201019)

摘　　要：本研究探讨应用荧光染色试剂Calcofluor White M2R染色鉴别家蚕微孢子虫Nosema bombycis。结果表明:在荧光显微镜下可见家蚕微孢子虫孢子被染上强烈的青蓝色荧光,而寄主组织碎片、病毒、细菌等不被染色。该法是一种快速有效鉴别微孢子虫的方法。The fluorescent dye Calcofluor White M2R was applied to stain and identify Nosema bombycis spores. The results showed that the spores were stained in green-blue fluorescence. With this method it is easy to distinguish microsporidian spores from virus, bacteria and fungus spores.

关 键 词：家蚕微孢子虫 CALCOFLUOR WHITE M2R 荧光染色 识别技术 

分 类 号：Q965[生物学—昆虫学]