铬对烟草组培苗生长和某些生理指标的影响  

Effects of Cr^(6+) on the growth and some physiological characters of tobacco by tissue culture

作　　者：石贵玉[1] 秦丽凤[1] 陈耕云[1] 

机构地区：[1]广西师范大学生命科学学院,广西桂林541004

出　　处：《广西植物》2007年第6期899-902,共4页Guihaia

基　　金：广西教育厅科研基金~~

摘　　要：以烟草无菌苗为材料,在培养基中添加0、50、100、150、200、300μmol.L-1浓度的重金属铬离子,进行组织培养,35d后分析植株生长和相关生理变化。结果表明,低浓度(50μmol.L-1)铬对烟草组培苗生长有促进作用,株高、鲜重、叶绿素含量、蛋白质含量、SOD活性呈现上升趋势;高浓度铬处理则抑制生长,株高、鲜重、叶绿素、蛋白质含量、SOD活性都下降,相反,POD活性、MDA含量和细胞膜透性显著增加。After germfree tobacco seedlings being cultivated in culture medium added with Cr^6 ＋ solution in 0,50,100, 150,200,300 μmol · g^-1 consistency respectively for 35 days, the growth and certain physiological functions of the tobacco were studied. The results indicated： （1）the callus and seedlings growth was promoted at 50 μmol · L^-1 consistency,and the height, fresh weight, content of chlorophyll and protein, SOD（superoxide dismutase）activity of the seedlings increased; （2）the growth of callus and seedling was restrained distinctly under the high consistency （100- 300 μmol · k^-1 ）, and the seedling height, fresh weight, content of chlorophyll and protein, activity of SOD（superoxide dismutase）decreased; （3）on the contrary, the activity of peroxidase（POD）, content of MDA and the permeability of cell membrane had earned a remarkable increase with the high concentration of Cr^6＋.

关 键 词：铬 烟草 组织培养 生长生理 

分 类 号：Q945[生物学—植物学]