蜂毒疏水性短肽增强聚乙烯亚胺的基因转染效率  

Enhancing Transfection Efficiency of Polyethylenimine by a Hydrophobic Peptide from Bee Venom

作　　者：王颖丽[1] 章扬培[1] 季守平[1] 

机构地区：[1]军事医学科学院输血医学研究所,北京100850

出　　处：《中国实验血液学杂志》2007年第6期1266-1269,共4页Journal of Experimental Hematology

摘　　要：本研究探讨使用蜂毒短肽疏水性尾部增加支链聚乙烯亚胺(BPEI)在HeLa细胞中的转染效率的可能性。合成蜂毒多肽melittin的疏水性尾部,使用红细胞溶血实验检测其穿透细胞膜的能力。将该片段与阳离子聚合物BPEI按一定比例混合,以绿色荧光蛋白(GFP)为报告基因,检测其在HeLa细胞的转染效率;以MTT法检测基因转染后的细胞毒性。结果表明:在中性和酸性条件下,melittin的疏水性尾部均可穿透细胞膜导致红细胞溶血并显著增加BPEI在HeLa细胞系的基因转染效率,使用本方法转染后细胞毒性较单独使用PEI时为低。结论:melittin疏水性尾部是一种良好的增强剂,有可能在难以转染的细胞系的基因转染实验中起重要作用。The study was aimed to investigate the possibility of enhancing transfection efficiency of branched polyethylenimine （BPEI） in HeLa cells by hydrophobic tail of bee venom peptide （melittin）. Hydrophobic tail of melittin was synthesized and its membrane permeable activity was evaluated by hemolysis test. The peptide was mixed with BPEI and the transfection efficiency was determined in HeLa cells by using green fluorescent protein gene （GFP） as a reporter gene. The cytotoxicity of the mixture was analysized by MTT assay at 24 hours after transfection. The results indicated that the synthesized peptide had permeable activity leading to hemolysis in both neutral and acidic solution. At optimal condition, the peptide could significantly improve the transfection efficinecy of BPEI and the cytotoxicity of the mixture was lower than BPEI itself. It is concluded that hydrophobic tail of melittin may be a potential enhancer to improve transfection efficiency mediated by cationic polymers in difficult to transfect cells.

关 键 词：蜂毒多肽 阳离子聚合物 转染效率 细胞毒性 

分 类 号：R996.3[医药卫生—毒理学] Q753[医药卫生—药学]