Akt对哮喘小鼠下呼吸道及内脏感觉传入部位IL-1β表达的影响  

The Upregulatory Effect of PKB/Akt on IL-1β in the Lower Respiratory Tract and Viscerosensory Afferent Sites of the Asthmatic Mice

作　　者：张宝辉[1] 刘玉丽[1] 高鸣铎 于希富[3] 张正洪[4] 王平[3] 方秀斌[1] 

机构地区：[1]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001 [2]沈阳惠民生物有限公司,辽宁沈阳110141 [3]沈阳中药制药有限公司,辽宁沈阳110044 [4]郧阳医学院解剖教研室,湖北十堰市442000

出　　处：《解剖科学进展》2007年第4期289-292,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.30570811)

摘　　要：目的探讨在哮喘发病机制中,PKB/Akt对哮喘小鼠肺组织及C7-T5段脊神经节和相应节段脊髓后角IL-1β表达的调节作用。方法BALB/c小鼠30只,按随机数字表法均分为正常对照组、哮喘组、PKB/Akt阻断组,免疫组织化学检测各组小鼠肺组织及C7-T5节段脊神经节和相应节段脊髓后角IL-1β的表达,Western blot方法检测各组小鼠肺组织及C7-T5节段脊神经IL-1β的表达。结果免疫组织化学结果显示,哮喘组肺、C7-T5段脊神经节和相应节段脊髓后角IL-1β阳性产物的平均光密度值(MOD)显著高于正常对照组(P<0.01);而PKB/Akt阻断组与哮喘组相比MOD值明显降低(P<0.05)。Western blot结果显示:与正常对照组比较,哮喘组小鼠肺、C7～T5段脊神经中IL-1β的IDV(integrated density value)与β-actin IDV的比值均明显升高(P<0.01),而PKB/Akt阻断组明显低于哮喘组(P<0.05)。结论NGF介导的Akt通路的激活可上调IL-1β的表达,介导哮喘气道高反应和炎症反应。Objective To study the regulatory effect of PKB/Akt on the expression of IL-1β in the lung, C7 - T5 spinal ganglia and the corresponding spinal dorsal horn of the asthmatic mice. Methods 30 BALB/c mice were randomly divided into normal control group, asthmatic group, the asthmatic mice blocked with LY294002 group on average. The expression of IL-1β was observed by immunohistochemical method and Western blot. Results Immunohistochemistry showed that the mean optic density（MOD） of IL-1β positive product in the lung, spinal ganglia and dorsal horn of C7 - T5 increased significantly in the asthmatic mice than in normal control （ P 〈 0,01）, but decreased in the asthmatic mice blocked with LY294002 than in the asthmatic mice （ P 〈 0.05）. Western blot showed that the ratios of IL-1β integrated density value （ IDV）/β -actin IDV in the lung, C7 - T5 spinal cord increased significantly in the asthmatic mice than in normal control （ P 〈 0.01）, but decreased in the asthmatic mice blocked with LY294002 than in the asthmatic mice （ P 〈 0.05）. Conclusion The upregulation of IL-1β by the activated NGF-mediated Akt pathway might he involved in the asthmatic airway hyperreactivity and inflammation.

关 键 词：白介素1-β PKB/AKT 神经生长因子 哮喘 下呼吸道 脊神经节 脊髓后角 

分 类 号：Q189[生物学—神经生物学] R331[生物学—普通生物学]