锌离子和锌转运蛋白3在小鼠海马苔藓纤维的共存  

The Colocolization of Zinc and ZNT3 in Mossy Fibers of Mice Hippocampus

作　　者：刘冬娟[1] 徐彦平[1] 池志宏[2] 牛犁[2] 王月[2] 王占友[2] 

机构地区：[1]中国医科大学电子显微学研究室,辽宁沈阳110001 [2]中国医科大学组织胚胎学教研室,辽宁沈阳110001

出　　处：《解剖科学进展》2007年第4期338-339,共2页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.30370452)

摘　　要：目的研究锌离子与锌转运蛋白3(ZNT3)抗体进行双标的可行性。方法应用TSQ荧光技术以及普通荧光技术在小鼠海马苔藓纤维双重标记锌离子和ZNT3蛋白。结果在小鼠海马苔藓纤维,锌离子和ZNT3蛋白被各自标记,荧光反应阳性。而合并后的图片表明,两者实现完全共区域化。结论TSQ作为一种锌离子特殊荧光染色是可以和普通荧光染色的抗体进行双重标记的。Objective To investigate the possibility of double labeling for zinc and ZNT3 antibody. Method Double labeling for zinc and ZNT3 in mouse mossy fibers was performed by TSQ fluorescence and common fluorescence. Results Under the fluorescence microscope, dual immunofluo^scent labeling for ZNT3 and Zinc was observed with abundant labeling for both staining in mouse mossy fibers of hippocampus in mice. After merging the ZNT3- and TSQ-labeled images from the two channels, a correspondent colocalization for ZNT3 and TSQ could be demonstrated. Conclusion TSQ fluorescence and common fluorescence can be used for dual immunofluorescence stainning.

关 键 词：游离锌离子 N-(6-methoxy-8-quinolyl)-P-toluene-sulfonamide(TSQ)荧光技术 苔藓纤维 锌转 运蛋白3 

分 类 号：R338.26[医药卫生—人体生理学]