硒酸锌金属自显影技术检测游离锌离子在小鼠卵巢的分布  

The Distribution of Free Zinc Ions in Mouse Ovaries by Zinc Selenide Autometallography

作　　者：张莉[1] 池志宏[1] 王月[1] 牛犁[1] 王占友[1] 

机构地区：[1]中国医科大学基础医学院组织胚胎学教研室

出　　处：《解剖科学进展》2007年第4期346-347,352,共3页Progress of Anatomical Sciences

基　　金：国家自然科学基金资助项目(No.30370452)

摘　　要：目的研究游离锌离子在小鼠卵巢的定位分布。方法应用硒酸锌金属自显影技术(ZnSeAMG)检测小鼠卵巢内的游离锌离子分布。结果游离锌离子在卵巢内分布广泛,在原始卵泡、初级卵泡、次级卵泡、闭锁卵泡以及间质细胞中都有大量棕黑色的AMG染色颗粒。结论小鼠卵巢内含有丰富的游离锌离子,锌离子可能参与了卵泡的发育以及性激素的合成。Objective To investigate the localization of free zinc ions in mouse ovaries. Method Zinc selenide autometallography （ZnSe^AMG） was used to detect free zinc ions in mouse ovaries. Results Extensive AMG staining was observed all over the ovaries, including the primordial follicles, primary follicles, secondary follicles, atretic follicles and interstitial tissue of ovaries. Conclusion Lots of free zinc ions in ovaries may be involved in the development of the follicles and the synthesis of sex hormone.

关 键 词：游离锌离子 硒酸锌金属自显影技术 卵巢 

分 类 号：R335.5[医药卫生—人体生理学]