天麻素对快速衰老小鼠大脑组织衰老相关基因表达的影响  

Effect of Gastrodin on Aging-associated Genes Expression in Brain of Senescence-Accelerated Mice

作　　者：王昭君[1] 习杨彦彬[1] 刘佳[1] 戴萍[1] 冯忠堂[1] 王廷华[1] 

机构地区：[1]昆明医学院神经科学研究所,云南昆明650031

出　　处：《解剖科学进展》2007年第4期353-357,共5页Progress of Anatomical Sciences

基　　金：云南省国际合作项目资助(2005GH23)

摘　　要：目的研究天麻素对快速衰老小鼠(SAM)海马及其大脑额叶皮质中衰老相关基因表达的影响。方法采用RT-PCR方法对给予天麻素的SAM-P/8小鼠海马及额叶皮质中衰老相关基因的mRNA表达变化进行分析。结果所检测的衰老相关基因在SAM-P/8小鼠的海马和额叶皮质中均有表达。成年鼠应用天麻素组与成年组比较,在海马组织中SCN2BmRNA表达增加,Sortilin-1mRNA表达减少,MAP1BmRNA和RAP2AmRNA在额叶皮质中表达增加。老年鼠天麻素组MAPKK4、Sortilin-1和Rab6A在海马和额叶皮质中表达均比老年组减少。结论天麻素在分子水平上可能是通过调节部分衰老相关基因的表达水平来发挥其抗衰老作用,但对于衰老晚期,基因调控抗衰老的作用不明显。Objective To study the effect of Gastrodin on expressions of aging-associated genes in hippocampus and frontal lobe of senescence -accelerated mice （SAM）. Methods The expressional changes of agingassociated gene mRNAs in hippocampus and frontal lobe of SAM were analyzed by RT-PCR. Results All the determined aging-associated genes were detected in hippocampus and frontal lobe of SAM.The expression of SCN2B mRNA in hippocampus of adult mouse increased following Gastrodin administration, MAP1B mRNA and RAP2A mRNA expressions increased in frontal lobe, while the expression of Sortilin-1 mRNA decreased in hippocampus compared with the aging control.Compared with aging control, the expressions of MAPKK4, Sortilin-1 and Rab6A mRNA in Gastrodin treated group decreased.Conclusion Gastrodin could regulate the aging-associated genes expressions in brain of adult animal to be linked to anti-aging, but not for the senile advanced stage.

关 键 词：快速衰老小鼠 天麻素 衰老相关基因 逆转录聚合酶链式反应 脑组织 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]