用TK基因突变试验评价葛根素的抗诱变性  

Detection of Antimutagenicity of Puerarin by TK Gene Mutation Test

作　　者：丁晓琴[1] 陈杰[2] 张立实[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生教研室,成都610041 [2]西南财经大学,成都610074

出　　处：《癌变．畸变．突变》2007年第6期463-465,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(30471473)

摘　　要：背景与目的:用TK6人类淋巴母细胞TK基因突变试验评价葛根素的抗诱变性。材料与方法:设溶剂对照组、5μg/ml甲基磺酸甲酯(MMS)组、5μg/mlMMS分别加葛根素25μg/ml、50μg/ml、100μg/ml的各试验组、5μg/mlMMS+100μg/mlVitC对照组,共6组。按上述分组加入葛根素与MMS处理4h后测定第0d的平板接种效率(PE0)、第3d的平板接种效率(PE3)、细胞悬浮生长率(RSG)和总突变频率(TMF)。结果:100μg/ml葛根素剂量组能显著降低MMS诱导的TK基因突变频率。结论:在本实验条件下,葛根素在较高剂量范围可抑制MMS诱导的TK6细胞TK基因突变,有潜在的开发应用前景。BACKGROUND ＆ AIM： To detect and evaluate the MATERIALS AND METHODS： The TK6 human lymphoid cells were antimutagenicity of puerarin by TK gene mutation test. exposed to puerarin at concentrations of 25, 50, 100 μg/ml with 5 μg/ml MMS, 0.1% DMSO, 5 μg/ml MMS and 5 μg/ml MMS＋ 100 μg/ml VitC using simultaneous treatment to determine PE0, PE3, RSG and TMF. RFSULTS： Puerarin at concentration 100 μg/ml significantly decreased the TMF induced by MMS. CONCLUSION： Under this experimental condition,puerarin inhibited TK gene mutation on TK6 cell induced by MMS at higher concentration, and has Good Potential for further development and application.

关 键 词：TK基因突变试验 TK6细胞 葛根素 抗诱变性 

分 类 号：R994.6[医药卫生—毒理学]