金纳米粒子与谷胱甘肽相互作用及其分析应用  

Study on Interaction Between Gold Nanoparticles and Glutathione and Its Analytical Application

作　　者：许文杰[1] 李正平[2] 段新瑞[2] 秦磊[2] 

机构地区：[1]河北大学医学部,河北保定071000 [2]河北大学化学与环境科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2007年第3期265-269,共5页Journal of Hebei University(Natural Science Edition)

基　　金：教育部自然科学基金重点项目(205020)

摘　　要：通过谷胱甘肽的巯基（-SH）与金纳米粒子的共价结合和氨基（-NH3^＋）与金纳米粒子的静电作用,使金纳米粒子自组装为有序的网状超分子结构,导致金纳米粒子的最大吸收波长从520 nm红移到668 nm,且在668 nm处的吸光度与谷胱甘肽的浓度在一定范围内呈正比.由此建立了以金纳米粒子为探针,简便、灵敏的测定谷胱甘肽的分析方法.本方法线性范围为0.01-0.20 mg/L,检出限3.0μg/L（3σ,9.8 nmol/L）.Through the covalent combination with the - SH group and the electrostatic interaction with the - NH3^＋ group of glutathione, gold nanoparticles can self-assemble and form a supermolecular network structure, As a result, absorption peak of gold nanoparticles shifts from 520 nm to 668 nm, and the absorbance of gold nanoparticles at 668 nm is proportional to glutathione concentration in a certain range. Based on this study, a rapid, simple, sensitive method for glutathione determination using gold nanoparticles as a probe has been established. There is a good linear relationship between the absorbance at 668 nm and glutathione concentration in the range from 0.01 mg/L to 0.20 mg/L, the corresponding detection limit is 3.0 μg/L （3 a, 9.8 nmol/L）.

关 键 词：金纳米粒子 谷胱甘肽 紫外可见分光光度法 

分 类 号：O657.31[理学—分析化学]