眩晕定方对血管性眩晕模型家兔体重及肝组织DNA含量的实验研究  

Effect of Xuan Yun Ding on the Weight and the DNA Content of the Hepatic Tissue of Rabbits with Vascular Dizziness of Qi Deficiency and Blood Stasis Pattern

作　　者：黄云峰[1] 王净净[2] 刘春华[3] 姚雪祥[2] 

机构地区：[1]湖南省长沙市中心医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410007 [3]湖南中医药大学附属中西医结合医院,湖南长沙410006

出　　处：《世界中西医结合杂志》2007年第3期149-151,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：湖南省教育厅资助项目(No01C235);湖南省卫生厅资助项目(NoC-050425)

摘　　要：目的:研究眩晕定方对血管性眩晕气虚血瘀证模型家兔干预后,家兔体重及肝组织DNA含量的变化。方法:建立实验性血管性眩晕气虚血瘀证家兔模型,采用苯酚法提取兔肝DNA,紫外吸收法测定其DNA含量,观察眩晕定方干预后家兔体重及肝组织DNA含量的改变情况。结果:眩晕定方作用于气虚血瘀型血管性眩晕动物模型后,中剂量组体重为(2.54±0.13)kg,高于西比灵对照组的(2.06±0.10)kg,差异有显著统计学意义(P<0.01);中剂量组肝组织DNA含量为(1.1029±0.0916)kg,低于于西比灵组的(1.4236±0.1115)kg,差异有显著统计学意义(P<0.01)。结论:眩晕定方可增加家兔体重,降低该模型家兔的肝组织DNA含量,从而改善气虚证候。Objective： To observe the changes of the weight and the DNA content of the hepatic tissue of the rabbits with vascular dizziness of qi deficiency and blood stasis pattern treated by Xuan Yun Ding. Methods： An experimental animal vascular dizziness of qi deficiency and blood stasis model was built in rabbits, the hepatic tissue DNA of the rabbits were extracted with carbolic acid, and their content was determined with ultraviolet absorption method. Results： The weight of rabbits in the middle-dosage Xuan Yun Ding group was 2.54±0.13 kg , high than that of control group treated with Flunarizine（2.06 ±0.10）kg with remarkable significance （P〈0.01） ; the DNA content was 1. 1029±0. 0916kg, lower than that in the control group （1. 4236±0. 1115） kg with a notable significance（P〈0.01）. Conclusion： The formula of Xuan Yun Ding may increase the weight of rabbits, decrease the hepatic tissue DNA content and improve the condition of qi deficiency syndrome.

关 键 词：眩晕定方 血管性眩晕 气虚血瘀 动物模型 家兔 肝DNA含量 

分 类 号：R255.3[医药卫生—中医内科学]