正常与性早熟河蟹视神经节蛋白的二维电泳初步分析  

Protein Analysis of Optic Ganglia of Sex Precocity and Mature Eriocheir sinensis by Two-dimensional Electrophoresis

作　　者：李云峰[1] 康现江[1] 温秀荣[1] 穆淑梅[1] 赵晓瑜[1] 张亮[1] 

机构地区：[1]河北大学生命科学学院

出　　处：《河北大学学报（自然科学版）》2007年第3期300-303,共4页Journal of Hebei University(Natural Science Edition)

基　　金：国家自然科学资金资助项目(30371115);河北省自然科学基金资助项目(303118)

摘　　要：利用双向电泳技术比较正常和性早熟河蟹视神经节蛋白的表达差异,旨在探索蛋白质组学技术在甲壳动物中尤其是河蟹的性早熟研究当中的应用.结果发现,明显差异的蛋白点共24个,为以后通过蛋白质组学手段筛选出真正影响性早熟发生的蛋白质或酶奠定了基础.The protein expression of normal and precocity E. sinensis＇s optic ganglia was compared by 2- DE. From the 2-DE maps, 115 protein points of normal crab and 108 of precocity crab were found . The result indicates that there are 24 obviously different protein points, and more acidic proteins in precocity crab＇s ganglia than the normal. The different proteins were considered to be the special proteins concerned with the development of crab＇s gonad.

关 键 词：双向电泳 性早熟 河蟹 视神经节 

分 类 号：Q958.8[生物学—动物学]