血管紧张素转换酶基因多态性与老年人心脑血管病的相关性研究  

ACE polymorphism analyzed in aged patients with heart and brain diseases

作　　者：顾国浩[1] 史进方[1] 蒲剑宏[2] 卢莉莉[3] 

机构地区：[1]苏州大学附属第一医院检验科,江苏苏州215006 [2]苏州大学附属第一医院老年病科,江苏苏州215006 [3]南京医科大学检验系,南京210029

出　　处：《临床检验杂志》2007年第6期413-415,共3页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省临床免疫学重点实验室基金项目(NO200319)。

摘　　要：目的探讨血管紧张素转换酶(ACE)基因多态性与老年人心脑血管病之间的相关性。方法用PCR、LDR及基因芯片技术对150例心脑血管病及126例正常对照组外周血白细胞作ACE基因多态性分析。结果ACED等位基因频率在老龄正常对照组及年轻对照组之间,在冠心病组与老龄正常对照组及年轻正常对照组之间均无显著性差别(P>0.05);高血压患者ACED/D基因型、脑梗死及脑萎缩组患者的ACED/D基因型与D等位基因频率均高于老龄正常对照组及年轻正常对照组(P<0.05与P<0.01)。结论ACED/D基因型可能是老年人高血压、脑梗死及脑萎缩的危险因素。Objective To investigate the contribution of angiotensin converting enzyme （ACE） polymorphism in aged patients with heart and brain diseases. Methods All the 276 subjects （ 126 healthy controls,150 patients with heart and brain diseases） underwent the determination of ACE polymorphism from leukocyte DNA by polymerase chain reaction （PCR）, ligase detection reaction （LDR） and gene chip. Results For ACE D/D genotype, no significant difference was observed between aged and young healthy groups （P 〉 0.05 ）, aged or young healthy control group and coronary heart disease group （P 〉 0.05 ）, but difference was existed between essential hypertension group and aged or young healthy control group （P 〈 0.05 ）. Significant differences were also found between aged or young healthy control group and brain infarct group as well as brain atrophy group（P 〈0.01 ）. Conclusion ACE D/D genotype may be the risk factor of essential hypertension, brain infarct and brain atrophy.

关 键 词：ACE基因多态性 冠心病 原发性高血压 脑梗死 脑萎缩 

分 类 号：R54[医药卫生—心血管疾病]