黄芩苷对体外培养神经干细胞分化的影响  

Effect of baicalin on differentiation of neural stem cells in vitro

作　　者：张密霞[1] 李越[1] 杜嵘[1] 张艳军[1] 

机构地区：[1]天津中医药大学中药学院,省部共建教育部重点方剂学实验室,300193

出　　处：《天津中医药大学学报》2007年第4期199-201,共3页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：国家自然基金项目资助(30472177)。

摘　　要：[目的]探索黄芩苷对体外神经干细胞分化的影响。[方法]培养胎鼠脑皮质神经干细胞,应用细胞免疫化学方法,以神经元特异性的微管相关蛋白-2(MAP-2)和星形胶质细胞的胶质纤维酸性蛋白(GFAP)为标记,对分化后的神经干细胞进行区分,评价黄芩苷对神经干细胞分化的影响。[结果]黄芩苷可以促进神经干细胞向神经元分化,其中高剂量组明显优于对照组。[结论]黄芩苷具有促进神经干细胞分化为神经元的作用。[Objective] To study the effect of Baicalin on the differentiation of neural stem cells （NSC）. [Methods] The NSC of rats was cultured in vitro. Cultured cells with Baiealin was pro- cessed for the immunoeytoehemistry assay. We detected the expression of the MAP-2 and GFAP, the makers of neuron and astroeytes. The effect of Baiealin on the differentiation of neural stem eells（NSC） was evaluated. [Results] Baiealin-treated NSC could develop mostly into neurons. This effeeting was depended on the used dose of Baiealin. The high dosage of baiealin group had an more obvious advantage than that of control group. [Conclusion] Baiealin has an effect on promoting differentiation of neural stem cells to neuron.

关 键 词：神经干细胞 黄芩苷 神经元分化 

分 类 号：R285.6[医药卫生—中药学]