CO对小鼠小脑基因表达的影响  

Effects of Carbon Monoxide on Cerebella Gene Expression in Mice.

作　　者：李曙光[1] 梁宏[1] 张恒太[1] 何新星[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《航天医学与医学工程》2007年第6期418-421,共4页Space Medicine & Medical Engineering

基　　金：中国载人航天工程基金资助

摘　　要：目的探讨CO对小鼠小脑基因表达的影响。方法利用差异表达基因克隆技术抑制消减杂交法(suppression subtractive hybridization,SSH),筛选750mg/m3CO暴露染毒72h小鼠小脑差异表达基因。结果获得了750mg/m3CO暴露染毒72h小鼠小脑差异表达的cDNA片段20个,克隆化后测序。结论CO主要影响细胞信号和传递、代谢、精子生成、免疫等功能相关的基因表达。Objective To investigate the effect of carbon monoxide （CQ） on cerebella gene expression in mice. Methods Suppression subtractive hybridization（SSH） technique was applied to screen differentially expressed gene in cerebella of mice breathed 750 mg/m^3 CO for 72 h. Results Twenty differentially expressed sequence tags found after exposed in 750 mg/m^3 CO for 72 h were cloned and determined. Conclusion CO toxicity may influence gene expression related to significant cell signs, transduction, metabolism, spermiogenesis and immune etc.

关 键 词：CO 小脑 基因表达 抑制消减杂交 

分 类 号：R852.17[医药卫生—航空、航天与航海医学]