dsRNA介导的PRSV-CP基因3′-端同源序列瞬时表达对番木瓜环斑病毒侵染的影响  

Transient Expression of dsRNA-mediated 3'-end Homologous Segment of PRSV-CP Gene and Influence with Virus Infection

作　　者：魏军亚[1] 刘德兵[1] 周鹏[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101

出　　处：《热带作物学报》2007年第3期78-82,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(30560082);国家转基因植物研究与产业化开发专项(JY04-B-02)资助

摘　　要：体外合成我国番木瓜主产区环斑病毒外壳蛋白(PRSV-CP)基因3'-端278bp同源区段,构建包含278bp正义链、内含子(PDK内含子)及278bp反义链的RNA介导的植物表达载体p2301-CPU,直接转化根癌农杆菌EHA105。通过根癌农杆菌介导的瞬时表达法,研究同源dsRNA对番木瓜环斑病毒(PRSV)侵染的干扰作用。结果表明,瞬时表达的dsRNA能够特异地干扰PRSV侵染。RNA-mediated resistance to virus infection in plant is associated with RNA silencing,in which double-strand RNA（dsRNA）induced sequence-specific post-transcriptional gene silencing.In this research,The 3＇-end homologous segment of coat protein gene of Papaya ringspot virus（PRSV）was cloned and the sense segment,antisense segment of the gene homologous segment of PRSV-CP and PDK intron segment were constructed into plant expression vector pCAMBIA2301 by using a series of cloning methods.The recombinant plasmid was transferred into competent cells of Agrobacterium tumefaciens EHA105.The interference effects of dsRNA targeting CP gene on PRSV infection were studied by Agrobacterium-mediated transient expression system.The results showed that transiently expressed dsRNA could specifically interfere with PRSV infection.

关 键 词：DSRNA 农杆菌浸润法 干扰 番木瓜环斑病毒 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]