IGF-I在大鼠肝肌纤维母细胞增殖中的作用研究  

The Effect of the insulin-I growth factor in rat liver myofibroblasts proliferation

作　　者：隋国德[1] 牛兆健[1] 胡三元[1] 张海峰[1] 张光永[1] 

机构地区：[1]山东大学齐鲁医院普外科,济南市250012

出　　处：《医学动物防制》2007年第7期481-484,共4页Journal of Medical Pest Control

摘　　要：目的研究胰岛素样生长因子-Ⅰ(IGF-在)在大鼠肝肌纤维母细胞(MF)增殖中的作用。方法培养1到4代的MF(P1-4)用于研究。用Northern blot RNA印迹杂交法测定IGF-ⅠmRNA的表达。5-溴-2-脱氧尿嘧啶核苷(BrdU)核素掺入法分析DNA合成。结果P1-4 MF表达了IGF-Ⅰ特有的mRNA的转录产物,IGF-Ⅰ和较少量的胰岛素能增加MF的DNA合成。IGF-Ⅰ与IGFBP-2或-3重组体同时作用能抑制IGF-Ⅰ刺激MF中DNA的合成。但是用IGFBP-2或-3对MF做预孵育后则进一步增强了IGF-Ⅰ刺激MF中DNA的合成。结论IGF-Ⅰ在MF增殖的调节中起了重要的作用,可能与急性和慢性肝损伤过程中发生的肝纤维化过程有密切的关系。Objective To study the effect of the IGF-Ⅰ in rat liver MF proliferation. Methods Cultures of MF from passages 1 to 4（P1-4）were studied. IGF-Ⅰ messenger RNA （mRNA）expression was assessed by Northern blot hybridization. DNA synthesis was evaluated by 5-bromo-2 -deoxyuridine （BrdU） incorporation assay. Results MF from P1 to 4 expressed mRNA transcripts specific for IGF-Ⅰ. IGF-Ⅰ and to a lesser extent insulin increased DNA synthesis of MF. Simultaneous addition of recombinant IGFBP-2 or -3 with IGF-Ⅰ diminished the mitogenic effect of IGF-Ⅰ on MF whereas preincubation of MF with IGFBP-2 or -3 further potentiated the IGF-Ⅰ stimulated DNA synthesis. Conclusion IGF-Ⅰ play an important role in the regulation of MF proliferation which might be relevant for the process of fibrogenesis during acute and chronic liver injury.

关 键 词：胰岛素样生长因子-Ⅰ 肝肌纤维母细胞 MRNA DNA 

分 类 号：R458.5[医药卫生—治疗学]