镍胁迫对大豆种子萌发过程中的生理生化影响  

Effects of nickel stress on the physiology and biochemistry aspects in the germination process of soybean seeds

作　　者：吴诗光[1] 

机构地区：[1]安庆师范学院生命科学系,安徽安庆246011

出　　处：《商丘师范学院学报》2007年第12期109-112,共4页Journal of Shangqiu Normal University

基　　金：河南省自然科学基金资助项目(编号:0411031500)

摘　　要：以两个大豆品种(周豆11号和豫豆24号)为材料,研究了不同浓度Ni2+处理对大豆种子萌发和幼苗生长中膜脂过氧化水平、细胞膜透性及保护酶(SOD、POD、CAT)活性的影响.结果表明,(1)Ni2+浸种处理对大豆种子萌发具有低浓度下的激活效应和高浓度下的抑制效应;(2)细胞内丙二醛(MDA)含量和细胞膜透性在低浓度Ni2+处理下略有降低,尔后随着镍浓度的提高其质膜透性和MDA含量逐渐增大,体内保护酶系统中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化表现为先升后降的趋势.不同大豆品种对Ni2+的反应存在差异,与抗氧化系统的整体活力有关.Employing two kinds of soybeans （Zhoudou 11 and Yudou 24 ） seeds as materials, Effects of nickel （Ni^2＋ ） treatment in different concentration on membrane lipid peroxidation level, membrane penetrability and activities of protective enzyme （SOD,POD, CAT） during seeds germination and seedlings growth were studied. The results show that the Ni^2＋ treatment has stimulant effect in low concentration but inhibitory effect in high concentration on soybean Seeds germination; Ni^2＋ concentration increase, the content of intra - cellular malondialdehyde （MDA） and the membrane penetrability slightly decrease at first, and then increase; the activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） in protective enzyme system exhibite the opposite tendency. Different soybeans have different reactions to Ni^2＋ , owing to their entire activity of antioxygen systems.

关 键 词：大豆 镍 保护酶 

分 类 号：Q945.14[生物学—植物学]