人混合脐血对SCID小鼠移植实验研究  

Studies on the engraftment of human mixed cord blood in severe combined immunodeficient mice

作　　者：张建忠[1,2] 吕善根[1,2] 裴雪涛[1,2] 王立生 张晓飞[1,2] 徐黎[1,2] 

机构地区：[1]解放军总医院小儿内科 [2]军事医学科学院放射医学研究所

出　　处：《中华血液学杂志》1997年第8期403-405,共3页Chinese Journal of Hematology

摘　　要：目的：探讨人混合脐血移植的特性。方法：将人单个脐血或两个ＨＬＡ不相合的混合脐血输入经亚致死剂量照射后的严重联合免疫缺陷（ＳＣＩＤ）小鼠，观察两组脐血在ＳＣＩＤ小鼠体内的植入率和ＳＣＩＤ小鼠的造血重建及免疫功能的特性。结果：在输入造血干／祖细胞总量相等时，两组移植成功率无明显差异（Ｐ＞０．０５）；混合脐血组的植入率较单个脐血组的低［人ＣＦＵ－ＧＭ为２．８０±１．７９／２×１０５ＭＮＣｓ比１３．４０±８．１４／２×１０５ＭＮＣｓ（Ｐ＜０．０５）；ＣＤ４５＋细胞为０．９１％±０．６０％比８．９６％±１．７９％（Ｐ＜０．０１）］；混合脐血组ＳＣＩＤ小鼠的淋巴细胞转化率也较低（４．００％±４．１８％比２４．６０％±１８．３２％，Ｐ＜０．０５）。用多聚酶链反应－寡聚核苷酸单链构象多态性检测人ＨＬＡ－ＤＰＢ基因发现，两个脐血混合移植只有１个脐血植入ＳＣＩＤ小鼠，该脐血含造血干／祖细胞数（ＣＦＵ－ＧＭ、ＣＤ３４＋细胞）较多。结论：ＨＬＡ不相合的两个脐血混合移植ＳＣＩＤ小鼠可以重建其造血和免疫功能，但仅有含造血干／祖细胞较多、增殖活性较高的那个脐血植入ＳＣＩＤ小鼠。Objective: To evaluate the engrafting characteristics of human mixed cord bloods.Methods:Single or double human cord bloods were transplanted into sublethally irradiated severe combined immunodeficient(SCID) mice.Engrafting rate,hematopoiesis reconstitution and immunologic functions between the two groups were compared.Results:The survival rates of both transplanted groups were equivalent(P>0.05)while the total volume of transfused cord bloods had no statistical differences.But the engrafting rate of mixed cord blood transplant(MCBT) group was lower than that of single cord blood transplant(SCBT) group.The lymphocyte transformation rate of MCBT group was also lower than that of SCBT group.In MCBT group,only one of the two mixed cord bloods was engrafted in SCID mice as shown by HLA DPB gene detection. Conclusion:Two HLA mismatched human cord bloods transplanted in SCID mice could reconstitute hematopoietic and immunologic functions,but only one cord blood with the higher proliferation potential was engrafted.

关 键 词：造血干细胞移植 混合脐血 联合免疫缺陷 

分 类 号：R593.305[医药卫生—内科学] R457.9[医药卫生—临床医学]